2w4x

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2w4x" [edit=sysop:move=sysop])
Current revision (15:43, 13 December 2023) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2w4x.png|left|200px]]
 
-
{{STRUCTURE_2w4x| PDB=2w4x | SCENE= }}
+
==BtGH84 in complex with STZ==
 +
<StructureSection load='2w4x' size='340' side='right'caption='[[2w4x]], [[Resolution|resolution]] 2.42&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2w4x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron_VPI-5482 Bacteroides thetaiotaomicron VPI-5482]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W4X FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.42&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=STZ:STREPTOZOTOCIN'>STZ</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w4x OCA], [https://pdbe.org/2w4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w4x RCSB], [https://www.ebi.ac.uk/pdbsum/2w4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w4x ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/OGA_BACTN OGA_BACTN] Biological function unknown. Capable of hydrolyzing the glycosidic link of O-GlcNAcylated proteins.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w4/2w4x_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w4x ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Despite decades of its use in diabetes research, the mechanism of cytotoxicity of streptozotocin (STZ) toward pancreatic beta-islet cells has remained a topic of discussion. Although STZ toxicity is likely a function of its capacity to promote DNA alkylation, it has been proposed that STZ induces pancreatic beta-cell death through O-GlcNAcase inhibition. In this report, we explore the binding mode of STZ to a close homolog of human O-GlcNAcase, BtGH84 from Bacteroides thetaiotaomicron. Our results show that STZ binds in the enzyme active site in its intact form, without the formation of a covalent adduct, consistent with solution studies on BtGH84 and human O-GlcNAcase, as well as with structural work on a homolog from Clostridium perfringens. The active site of the BtGH84 is considerably deformed upon STZ binding and as a result the catalytic machinery is expelled from the binding cavity.
-
===BTGH84 IN COMPLEX WITH STZ===
+
Structural insight into the mechanism of streptozotocin inhibition of O-GlcNAcase.,He Y, Martinez-Fleites C, Bubb A, Gloster TM, Davies GJ Carbohydr Res. 2009 Mar 31;344(5):627-31. Epub 2008 Dec 13. PMID:19217614<ref>PMID:19217614</ref>
-
{{ABSTRACT_PUBMED_19217614}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 2w4x" style="background-color:#fffaf0;"></div>
-
[[2w4x]] is a 1 chain structure of [[Beta-Hexosaminidase]] with sequence from [http://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W4X OCA].
+
==See Also==
==See Also==
*[[Beta-Hexosaminidase|Beta-Hexosaminidase]]
*[[Beta-Hexosaminidase|Beta-Hexosaminidase]]
-
 
+
*[[Beta-Hexosaminidase 3D structures|Beta-Hexosaminidase 3D structures]]
-
==Reference==
+
*[[O-GlcNAcase|O-GlcNAcase]]
-
<ref group="xtra">PMID:019217614</ref><references group="xtra"/>
+
== References ==
-
[[Category: Bacteroides thetaiotaomicron]]
+
<references/>
-
[[Category: Beta-N-acetylhexosaminidase]]
+
__TOC__
-
[[Category: Bubb, A.]]
+
</StructureSection>
-
[[Category: Davies, G J.]]
+
[[Category: Bacteroides thetaiotaomicron VPI-5482]]
-
[[Category: He, Y.]]
+
[[Category: Large Structures]]
-
[[Category: Martinez-Fleites, C.]]
+
[[Category: Bubb A]]
-
[[Category: Complex]]
+
[[Category: Davies GJ]]
-
[[Category: Glycosidase]]
+
[[Category: He Y]]
-
[[Category: Glycoside hydrolase]]
+
[[Category: Martinez-Fleites C]]
-
[[Category: Hydrolase]]
+
-
[[Category: Inhibitor]]
+

Current revision

BtGH84 in complex with STZ

PDB ID 2w4x

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2w4x"
Personal tools