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2l0i

From Proteopedia

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[[Image:2l0i.png|left|200px]]
 
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{{STRUCTURE_2l0i| PDB=2l0i | SCENE= }}
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==Solution structure of Rtt103 CTD-interacting domain bound to a Ser2 phosphorylated CTD peptide==
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<StructureSection load='2l0i' size='340' side='right'caption='[[2l0i]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2l0i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L0I FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2km4|2km4]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RTT103, YDR289C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l0i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0i OCA], [https://pdbe.org/2l0i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l0i RCSB], [https://www.ebi.ac.uk/pdbsum/2l0i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l0i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/RT103_YEAST RT103_YEAST]] Involved in transcription termination by RNA polymerase II and in regulation of Ty1 transposition.<ref>PMID:11779788</ref> <ref>PMID:15565157</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l0/2l0i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2l0i ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phosphorylation of the C-terminal domain (CTD) of RNA polymerase II controls the co-transcriptional assembly of RNA processing and transcription factors. Recruitment relies on conserved CTD-interacting domains (CIDs) that recognize different CTD phosphoisoforms during the transcription cycle, but the molecular basis for their specificity remains unclear. We show that the CIDs of two transcription termination factors, Rtt103 and Pcf11, achieve high affinity and specificity both by specifically recognizing the phosphorylated CTD and by cooperatively binding to neighboring CTD repeats. Single-residue mutations at the protein-protein interface abolish cooperativity and affect recruitment at the 3' end processing site in vivo. We suggest that this cooperativity provides a signal-response mechanism to ensure that its action is confined only to proper polyadenylation sites where Ser2 phosphorylation density is highest.
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===Solution structure of Rtt103 CTD-interacting domain bound to a Ser2 phosphorylated CTD peptide===
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Cooperative interaction of transcription termination factors with the RNA polymerase II C-terminal domain.,Lunde BM, Reichow SL, Kim M, Suh H, Leeper TC, Yang F, Mutschler H, Buratowski S, Meinhart A, Varani G Nat Struct Mol Biol. 2010 Oct;17(10):1195-201. Epub 2010 Sep 5. PMID:20818393<ref>PMID:20818393</ref>
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{{ABSTRACT_PUBMED_20818393}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2l0i" style="background-color:#fffaf0;"></div>
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[[2l0i]] is a 2 chain structure of [[RNA polymerase]] with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0I OCA].
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==See Also==
==See Also==
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*[[RNA polymerase|RNA polymerase]]
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020818393</ref><references group="xtra"/>
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__TOC__
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[[Category: Saccharomyces cerevisiae]]
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</StructureSection>
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[[Category: Buratowski, S.]]
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[[Category: Atcc 18824]]
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[[Category: Kim, M.]]
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[[Category: Large Structures]]
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[[Category: Leeper, T C.]]
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[[Category: Buratowski, S]]
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[[Category: Lunde, B M.]]
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[[Category: Kim, M]]
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[[Category: Meinhart, A.]]
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[[Category: Leeper, T C]]
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[[Category: Mutschler, H.]]
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[[Category: Lunde, B M]]
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[[Category: Reichow, S L.]]
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[[Category: Meinhart, A]]
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[[Category: Suh, H.]]
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[[Category: Mutschler, H]]
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[[Category: Varani, G.]]
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[[Category: Reichow, S L]]
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[[Category: Yang, F.]]
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[[Category: Suh, H]]
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[[Category: Varani, G]]
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[[Category: Yang, F]]
[[Category: 3' end processing]]
[[Category: 3' end processing]]
[[Category: Transcription]]
[[Category: Transcription]]

Current revision

Solution structure of Rtt103 CTD-interacting domain bound to a Ser2 phosphorylated CTD peptide

PDB ID 2l0i

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