1cq4

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[[Image:1cq4.png|left|200px]]
 
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{{STRUCTURE_1cq4| PDB=1cq4 | SCENE= }}
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==CI2 MUTANT WITH TETRAGLUTAMINE (MGQQQQGM) REPLACING MET59==
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<StructureSection load='1cq4' size='340' side='right'caption='[[1cq4]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1cq4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CQ4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cq4 OCA], [https://pdbe.org/1cq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cq4 RCSB], [https://www.ebi.ac.uk/pdbsum/1cq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cq4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ICI2_HORVU ICI2_HORVU] Inhibits both subtilisin and chymotrypsin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cq/1cq4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cq4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We have constructed mutants of chymotrypsin inhibitor 2 with short glutamine repeats inserted into its inhibitory loop. These mutants oligomerize when expressed in Escherichia coli. The dimer of a mutant with four glutamines now has been crystallized, and its structure has been solved by molecular replacement by using the wild-type monomer as a search model. The structure of each half of the dimer is found to be the same as that of the wild-type monomer, except around the glutamine insertion. It was proposed that the components of the oligomers are held together by hydrogen bonds between the main-chain and side-chain amides of the glutamine repeats. Instead, they appear to form by swapping domains on folding in E. coli, and the glutamine repeats connecting the components of the dimers are disordered.
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===CI2 MUTANT WITH TETRAGLUTAMINE (MGQQQQGM) REPLACING MET59===
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Crystal structure of a dimeric chymotrypsin inhibitor 2 mutant containing an inserted glutamine repeat.,Chen YW, Stott K, Perutz MF Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1257-61. PMID:9990011<ref>PMID:9990011</ref>
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{{ABSTRACT_PUBMED_9990011}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1cq4" style="background-color:#fffaf0;"></div>
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[[1cq4]] is a 2 chain structure of [[Chymotrypsin Inhibitor]] with sequence from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQ4 OCA].
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==See Also==
==See Also==
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*[[Chymotrypsin Inhibitor|Chymotrypsin Inhibitor]]
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*[[Chymotrypsin inhibitor 3D structures|Chymotrypsin inhibitor 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:009990011</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Hordeum vulgare]]
[[Category: Hordeum vulgare]]
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[[Category: Chen, Y W.]]
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[[Category: Large Structures]]
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[[Category: Stott, K R.]]
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[[Category: Chen YW]]
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[[Category: Hydrolase inhibitor]]
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[[Category: Stott KR]]
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[[Category: Immune system]]
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[[Category: Polyglutamine insertion mutant]]
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[[Category: Serine protease inhibitor]]
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[[Category: Subtilisin- chymotrypsin inhibitor-2]]
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Current revision

CI2 MUTANT WITH TETRAGLUTAMINE (MGQQQQGM) REPLACING MET59

PDB ID 1cq4

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