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1e00

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[[Image:1e00.gif|left|200px]]<br /><applet load="1e00" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1e00, resolution 1.83&Aring;" />
 
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'''PORCINE ODORANT BINDING PROTEIN COMPLEXED WITH 2,6-DIMETHYL-7-OCTEN-2-OL'''<br />
 
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==Overview==
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==Porcine Odorant Binding Protein Complexed with 2,6-dimethyl-7-octen-2-ol==
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Porcine odorant binding protein (pOBP) is a monomer of 157 amino acid residues, purified in abundance from pig nasal mucosa. In contrast to the observation on lipocalins as retinol binding protein (RBP), major urinary protein (MUP) or bovine odorant binding protein (bOBP), no naturally occurring ligand was found in the beta-barrel cavity of pOBP. Porcine OBP was therefore chosen as a simple model for structure/function studies with odorant molecules. In competition experiments with tritiated pyrazine, the affinity of pOBP towards several odorant molecules belonging to different chemical classes has been found to be of the micromolar order, with a 1:1 stoichiometry. The X-ray structures of pOBP complexed to these molecules were determined at resolution between 2.15 and 1.4 A. As expected, the electron density of the odorant molecules was observed into the hydrophobic beta-barrel of the lipocalin. Inside this cavity, very few specific interactions were established between the odorant molecule and the amino acid side-chains, which did not undergo significant conformational change. The high B-factors observed for the odorant molecules as well as the existence of alternative conformations reveal a non-specific mode of binding of the odorant molecules in the cavity.
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<StructureSection load='1e00' size='340' side='right'caption='[[1e00]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1e00]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E00 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.83&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DHM:2,6-DIMETHYL-7-OCTEN-2-OL'>DHM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e00 OCA], [https://pdbe.org/1e00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e00 RCSB], [https://www.ebi.ac.uk/pdbsum/1e00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e00 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/OBP_PIG OBP_PIG] This protein is found in nasal epithelium and it binds a wide variety of chemical odorants.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e0/1e00_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e00 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1E00 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=DHM:'>DHM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Dhm+Binding+Site+For+Chain+A'>AC1</scene> and <scene name='pdbsite=AC2:Dhm+Binding+Site+For+Chain+B'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E00 OCA].
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*[[Odorant binding protein 3D structures|Odorant binding protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Complexes of porcine odorant binding protein with odorant molecules belonging to different chemical classes., Vincent F, Spinelli S, Ramoni R, Grolli S, Pelosi P, Cambillau C, Tegoni M, J Mol Biol. 2000 Jun 30;300(1):127-39. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10864504 10864504]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Cambillau, C.]]
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[[Category: Cambillau C]]
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[[Category: Spinelli, S.]]
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[[Category: Spinelli S]]
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[[Category: Tegoni, M.]]
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[[Category: Tegoni M]]
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[[Category: Vincent, F.]]
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[[Category: Vincent F]]
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[[Category: DHM]]
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[[Category: lipocalins]]
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[[Category: odorant binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:22:19 2008''
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Current revision

Porcine Odorant Binding Protein Complexed with 2,6-dimethyl-7-octen-2-ol

PDB ID 1e00

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