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1e15

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[[Image:1e15.gif|left|200px]]<br /><applet load="1e15" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1e15, resolution 1.90&Aring;" />
 
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'''CHITINASE B FROM SERRATIA MARCESCENS'''<br />
 
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==Overview==
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==Chitinase B from Serratia Marcescens==
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In this paper, we describe the structure of chitinase B from Serratia marcescens, which consists of a catalytic domain with a TIM-barrel fold and a 49-residue C-terminal chitin-binding domain. This chitinase is the first structure of a bacterial exochitinase, and it represents one of only a few examples of a glycosyl hydrolase structure having interacting catalytic and substrate-binding domains. The chitin-binding domain has exposed aromatic residues that contribute to a 55-A long continuous aromatic stretch extending into the active site. Binding of chitin oligomers is blocked beyond the -3 subsite, which explains why the enzyme has chitotriosidase activity and degrades the chitin chain from the nonreducing end. Comparison of the chitinase B structure with that of chitinase A explains why these enzymes act synergistically in the degradation of chitin.
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<StructureSection load='1e15' size='340' side='right'caption='[[1e15]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1e15]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E15 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e15 OCA], [https://pdbe.org/1e15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e15 RCSB], [https://www.ebi.ac.uk/pdbsum/1e15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e15 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q54276_SERMA Q54276_SERMA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e1/1e15_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e15 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1E15 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E15 OCA].
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*[[Chitinase 3D structures|Chitinase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of a two-domain chitotriosidase from Serratia marcescens at 1.9-A resolution., van Aalten DM, Synstad B, Brurberg MB, Hough E, Riise BW, Eijsink VG, Wierenga RK, Proc Natl Acad Sci U S A. 2000 May 23;97(11):5842-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10823940 10823940]
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[[Category: Large Structures]]
[[Category: Serratia marcescens]]
[[Category: Serratia marcescens]]
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[[Category: Single protein]]
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[[Category: Brurberg MB]]
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[[Category: Aalten, D M.F Van.]]
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[[Category: Eijsink VGH]]
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[[Category: Brurberg, M B.]]
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[[Category: Hough E]]
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[[Category: Eijsink, V G.H.]]
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[[Category: Riise BW]]
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[[Category: Hough, E.]]
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[[Category: Synstad B]]
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[[Category: Riise, B W.]]
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[[Category: Van Aalten DMF]]
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[[Category: Synstad, B.]]
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[[Category: Wierenga RK]]
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[[Category: Wierenga, R K.]]
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[[Category: chitin degradation]]
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[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:22:42 2008''
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Current revision

Chitinase B from Serratia Marcescens

PDB ID 1e15

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