1e5u

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[[Image:1e5u.gif|left|200px]]<br /><applet load="1e5u" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1e5u" />
 
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'''NMR REPRESENTATIVE STRUCTURE OF INTIMIN-190 (INT190) FROM ENTEROPATHOGENIC E. COLI'''<br />
 
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==Overview==
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==NMR Representative Structure of Intimin-190 (Int190) from Enteropathogenic E. coli==
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Intimin is a bacterial adhesion molecule involved in intimate attachment of enteropathogenic and enterohaemorrhagic Escherichia coli to mammalian host cells. Intimin targets the translocated intimin receptor (Tir), which is exported by the bacteria and integrated into the host cell plasma membrane. In this study we localized the Tir-binding region of intimin to the C-terminal 190 amino acids (Int190). We have also determined the region's high-resolution solution structure, which comprises an immunoglobulin domain that is intimately coupled to a novel C-type lectin domain. This fragment, which is necessary and sufficient for Tir interaction, defines a new super domain in intimin that exhibits striking structural similarity to the integrin-binding domain of the Yersinia invasin and C-type lectin families. The extracellular portion of intimin comprises an articulated rod of immunoglobulin domains extending from the bacterium surface, conveying a highly accessible 'adhesive tip' to the target cell. The interpretation of NMR-titration and mutagenesis data has enabled us to identify, for the first time, the binding site for Tir, which is located at the extremity of the Int190 moiety.
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<StructureSection load='1e5u' size='340' side='right'caption='[[1e5u]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1e5u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1e1b 1e1b] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1inm 1inm]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E5U FirstGlance]. <br>
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1E5U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry 1E1B. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E5U OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e5u OCA], [https://pdbe.org/1e5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e5u RCSB], [https://www.ebi.ac.uk/pdbsum/1e5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e5u ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structural basis for recognition of the translocated intimin receptor (Tir) by intimin from enteropathogenic Escherichia coli., Batchelor M, Prasannan S, Daniell S, Reece S, Connerton I, Bloomberg G, Dougan G, Frankel G, Matthews S, EMBO J. 2000 Jun 1;19(11):2452-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10835344 10835344]
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== Function ==
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[https://www.uniprot.org/uniprot/EAE_ECO27 EAE_ECO27] Necessary for the production of attaching and effacing lesions on tissue culture cells. Believed to mediate adherence.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e5/1e5u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e5u ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Batchelor, M.]]
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[[Category: Batchelor M]]
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[[Category: Bloomberg, G.]]
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[[Category: Bloomberg G]]
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[[Category: Connerton, I.]]
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[[Category: Connerton I]]
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[[Category: Daniell, S.]]
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[[Category: Daniell S]]
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[[Category: Dougan, G.]]
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[[Category: Dougan G]]
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[[Category: Frankel, G.]]
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[[Category: Frankel G]]
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[[Category: Matthews, S J.]]
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[[Category: Matthews SJ]]
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[[Category: Prasannan, S.]]
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[[Category: Prasannan S]]
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[[Category: Reece, S.]]
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[[Category: Reece S]]
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[[Category: cell adhesion]]
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[[Category: escherichia coli]]
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[[Category: intimin]]
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[[Category: nmr spectroscopy]]
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[[Category: outer membrane protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:24:09 2008''
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Current revision

NMR Representative Structure of Intimin-190 (Int190) from Enteropathogenic E. coli

PDB ID 1e5u

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