2w0q

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[[Image:2w0q.png|left|200px]]
 
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{{STRUCTURE_2w0q| PDB=2w0q | SCENE= }}
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==E. coli copper amine oxidase in complex with Xenon==
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<StructureSection load='2w0q' size='340' side='right'caption='[[2w0q]], [[Resolution|resolution]] 2.48&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2w0q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W0Q FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.48&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=TPQ:5-(2-CARBOXY-2-AMINOETHYL)-2-HYDROXY-1,4-BENZOQUINONE'>TPQ</scene>, <scene name='pdbligand=XE:XENON'>XE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w0q OCA], [https://pdbe.org/2w0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w0q RCSB], [https://www.ebi.ac.uk/pdbsum/2w0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w0q ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AMO_ECOLI AMO_ECOLI] The enzyme prefers aromatic over aliphatic amines.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w0/2w0q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w0q ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The mechanism of molecular oxygen entry into the buried active site of the copper amine oxidase family has been investigated in several family members using biochemical, structural and in silico methods. These studies have revealed a structurally conserved beta-sandwich which acts as a hydrophobic reservoir from which molecular oxygen can take several species-specific preferred pathways to the active site. Escherichia coli copper amine oxidase (ECAO) possesses an extra N-terminal domain that lies close to one entrance to the beta-sandwich. In order to investigate whether the presence of this domain alters molecular oxygen entry in this enzyme, xenon was used as a molecular oxygen binding-site probe. The resulting 2.5 A resolution X-ray crystal structure reveals xenon bound in similar positions to those observed in xenon-derivative crystal structures of other family members, suggesting that the N-terminal domain does not affect oxygen entry and that the E. coli enzyme takes up oxygen in a similar manner to the rest of the copper amine oxidase family.
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===E. COLI COPPER AMINE OXIDASE IN COMPLEX WITH XENON===
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Structure of a xenon derivative of Escherichia coli copper amine oxidase: confirmation of the proposed oxygen-entry pathway.,Pirrat P, Smith MA, Pearson AR, McPherson MJ, Phillips SE Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Dec 1;64(Pt, 12):1105-9. Epub 2008 Nov 28. PMID:19052360<ref>PMID:19052360</ref>
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{{ABSTRACT_PUBMED_19052360}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2w0q" style="background-color:#fffaf0;"></div>
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[[2w0q]] is a 2 chain structure of [[Copper Amine Oxidase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W0Q OCA].
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==See Also==
==See Also==
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*[[Copper Amine Oxidase|Copper Amine Oxidase]]
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*[[Copper amine oxidase 3D structures|Copper amine oxidase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019052360</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Monoamine oxidase]]
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[[Category: Large Structures]]
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[[Category: McPherson, M J.]]
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[[Category: McPherson MJ]]
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[[Category: Pearson, A R.]]
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[[Category: Pearson AR]]
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[[Category: Phillips, S E.V.]]
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[[Category: Phillips SEV]]
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[[Category: Pirrat, P.]]
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[[Category: Pirrat P]]
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[[Category: Smith, M A.]]
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[[Category: Smith MA]]
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[[Category: Copper amine oxidase]]
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[[Category: E. coli]]
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[[Category: Metal-binding]]
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[[Category: Oxidoreductase]]
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[[Category: Oxygen entry]]
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[[Category: Tpq]]
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[[Category: Xenon]]
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Current revision

E. coli copper amine oxidase in complex with Xenon

PDB ID 2w0q

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