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1eg7

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[[Image:1eg7.jpg|left|200px]]<br /><applet load="1eg7" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eg7, resolution 2.50&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA'''<br />
 
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==Overview==
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==THE CRYSTAL STRUCTURE OF FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA==
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The structure was solved at 2.5 A resolution using multiwavelength anomalous dispersion (MAD) scattering by Se-Met residues. The subunit of N(10)-formyltetrahydrofolate synthetase is composed of three domains organized around three mixed beta-sheets. There are two cavities between adjacent domains. One of them was identified as the nucleotide binding site by homology modeling. The large domain contains a seven-stranded beta-sheet surrounded by helices on both sides. The second domain contains a five-stranded beta-sheet with two alpha-helices packed on one side while the other two are a wall of the active site cavity. The third domain contains a four-stranded beta-sheet forming a half-barrel. The concave side is covered by two helices while the convex side is another wall of the large cavity. Arg 97 is likely involved in formyl phosphate binding. The tetrameric molecule is relatively flat with the shape of the letter X, and the active sites are located at the end of the subunits far from the subunit interface.
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<StructureSection load='1eg7' size='340' side='right'caption='[[1eg7]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1eg7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EG7 FirstGlance]. <br>
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1EG7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Formate--tetrahydrofolate_ligase Formate--tetrahydrofolate ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.3 6.3.4.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG7 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eg7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eg7 OCA], [https://pdbe.org/1eg7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eg7 RCSB], [https://www.ebi.ac.uk/pdbsum/1eg7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eg7 ProSAT]</span></td></tr>
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The crystal structure of N(10)-formyltetrahydrofolate synthetase from Moorella thermoacetica., Radfar R, Shin R, Sheldrick GM, Minor W, Lovell CR, Odom JD, Dunlap RB, Lebioda L, Biochemistry. 2000 Apr 11;39(14):3920-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10747779 10747779]
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</table>
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[[Category: Formate--tetrahydrofolate ligase]]
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== Function ==
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[https://www.uniprot.org/uniprot/FTHS_MOOTH FTHS_MOOTH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/1eg7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eg7 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Moorella thermoacetica]]
[[Category: Moorella thermoacetica]]
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[[Category: Single protein]]
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[[Category: Dunlap RB]]
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[[Category: Dunlap, R B.]]
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[[Category: Lebioda L]]
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[[Category: Lebioda, L.]]
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[[Category: Lovell CR]]
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[[Category: Lovell, C R.]]
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[[Category: Minor W]]
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[[Category: Minor, W.]]
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[[Category: Odom JD]]
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[[Category: Odom, J D.]]
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[[Category: Radfar R]]
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[[Category: Radfar, R.]]
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[[Category: Sheldrick GM]]
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[[Category: Sheldrick, G M.]]
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[[Category: Shin R]]
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[[Category: Shin, R.]]
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[[Category: SO4]]
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[[Category: atp binding]]
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[[Category: folate binding]]
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[[Category: formate binding]]
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[[Category: monovalent cation binding]]
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[[Category: synthetase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:27:27 2008''
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Current revision

THE CRYSTAL STRUCTURE OF FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA

PDB ID 1eg7

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