1egk

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:02, 7 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1egk.gif|left|200px]]<br /><applet load="1egk" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1egk, resolution 3.1&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF A NUCLEIC ACID FOUR-WAY JUNCTION'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF A NUCLEIC ACID FOUR-WAY JUNCTION==
-
A crystal structure of a 108 nucleotide RNA-DNA complex containing a four-way junction was solved at 3.1 A resolution. The structure of the junction differs substantially from the "stacked-X" conformation observed previously, due to a 135 degrees rotation of the branches. Comparison of the two conformers provides insight into the factors contributing to the flexibility of four-way junctions. The stacked-X conformation maximizes base-stacking but causes unfavorable repulsion between phosphate groups, whereas the 135 degrees -rotated "crossed" conformation minimizes electrostatic clashes at the expense of reduced base-stacking. Despite the large rotation of the branches, both junction structures exhibit an antiparallel arrangement of the continuous strands and opposite polarity of the crossover strands.
+
<StructureSection load='1egk' size='340' side='right'caption='[[1egk]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1egk]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EGK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EGK FirstGlance]. <br>
-
1EGK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EGK OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1egk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1egk OCA], [https://pdbe.org/1egk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1egk RCSB], [https://www.ebi.ac.uk/pdbsum/1egk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1egk ProSAT]</span></td></tr>
-
Alternative conformations of a nucleic acid four-way junction., Nowakowski J, Shim PJ, Stout CD, Joyce GF, J Mol Biol. 2000 Jun 30;300(1):93-102. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10864501 10864501]
+
</table>
-
[[Category: Protein complex]]
+
__TOC__
-
[[Category: Joyce, G F.]]
+
</StructureSection>
-
[[Category: Nowakowski, J.]]
+
[[Category: Large Structures]]
-
[[Category: Shim, P J.]]
+
[[Category: Joyce GF]]
-
[[Category: Stout, C D.]]
+
[[Category: Nowakowski J]]
-
[[Category: MG]]
+
[[Category: Shim PJ]]
-
[[Category: 10-23 dna enzyme]]
+
[[Category: Stout CD]]
-
[[Category: four-way junction]]
+
-
[[Category: nucleic acid]]
+
-
[[Category: ribozyme]]
+
-
[[Category: rna-dna complex]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:27:34 2008''
+

Current revision

CRYSTAL STRUCTURE OF A NUCLEIC ACID FOUR-WAY JUNCTION

PDB ID 1egk

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1egk"
Personal tools