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1ei7

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[[Image:1ei7.jpg|left|200px]]<br /><applet load="1ei7" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ei7, resolution 2.45&Aring;" />
 
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'''TMV COAT PROTEIN REFINED FROM THE 4-LAYER AGGREGATE'''<br />
 
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==Overview==
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==TMV COAT PROTEIN REFINED FROM THE 4-LAYER AGGREGATE==
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Previous x-ray studies (2.8-A resolution) on crystals of tobacco mosaic virus coat protein grown from solutions containing high salt have characterized the structure of the protein aggregate as a dimer of a bilayered cylindrical disk formed by 34 chemically identical subunits. We have determined the crystal structure of the disk aggregate at 2.4-A resolution using x-ray diffraction from crystals maintained at cryogenic temperatures. Two regions of interest have been extensively refined. First, residues of the low-radius loop region, which were not modeled previously, have been traced completely in our electron density maps. Similar to the structure observed in the virus, the right radial helix in each protomer ends around residue 87, after which the protein chain forms an extended chain that extends to the left radial helix. The left radial helix appears as a long alpha-helix with high temperature factors for the main-chain atoms in the inner portion. The side-chain atoms in this region (residues 90-110) are not visible in the electron density maps and are assumed to be disordered. Second, interactions between subunits in the symmetry-related central A pair have been determined. No direct protein-protein interactions are observed in the major overlap region between these subunits; all interactions are mediated by two layers of ordered solvent molecules. The current structure emphasizes the importance of water in biological macromolecular assemblies.
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<StructureSection load='1ei7' size='340' side='right'caption='[[1ei7]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ei7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tobacco_mosaic_virus Tobacco mosaic virus]. The January 2009 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Tobacco Mosaic Virus'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2009_1 10.2210/rcsb_pdb/mom_2009_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EI7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ei7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ei7 OCA], [https://pdbe.org/1ei7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ei7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ei7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ei7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAPSD_TMV CAPSD_TMV] Capsid protein self-assembles to form rod-shaped virions about 18 nm in diameter with a central canal enclosing the viral genomic RNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/1ei7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ei7 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EI7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Tobacco_mosaic_virus Tobacco mosaic virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EI7 OCA].
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*[[Tobacco Mosaic Virus|Tobacco Mosaic Virus]]
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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==Reference==
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__TOC__
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Refined atomic model of the four-layer aggregate of the tobacco mosaic virus coat protein at 2.4-A resolution., Bhyravbhatla B, Watowich SJ, Caspar DL, Biophys J. 1998 Jan;74(1):604-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9449361 9449361]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Tobacco Mosaic Virus]]
[[Category: Tobacco mosaic virus]]
[[Category: Tobacco mosaic virus]]
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[[Category: Bhyravbhatla, B.]]
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[[Category: Bhyravbhatla B]]
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[[Category: Caspar, D L.]]
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[[Category: Caspar DL]]
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[[Category: Watowich, S J.]]
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[[Category: Watowich SJ]]
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[[Category: disordered loops]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:28:01 2008''
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Current revision

TMV COAT PROTEIN REFINED FROM THE 4-LAYER AGGREGATE

PDB ID 1ei7

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