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1eih

From Proteopedia

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[[Image:1eih.jpg|left|200px]]<br /><applet load="1eih" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eih" />
 
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'''SOLUTION STRUCTURE OF THE HUMAN CHEMOKINE EOTAXIN-2'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF THE HUMAN CHEMOKINE EOTAXIN-2==
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The human CC chemokine eotaxin-2 is a specific agonist for the chemokine receptor CCR3 and may play a role in the recruitment of eosinophils in allergic diseases and parasitic infections. We report the solution structure of eotaxin-2 determined using heteronuclear and triple resonance NMR methods. A family of 20 structures was calculated by hybrid distance geometry-simulated annealing from 854 NOE distance restraints, 48 dihedral angle restraints, and 12 hydrogen bond restraints. The structure of eotaxin-2 (73 amino acid residues) consists of a helical turn (residues 17-20) followed by a 3-stranded antiparallel beta-sheet (residues 22-26, 37-41, and 44-49) and an alpha-helix (residues 54-66). The N-loop (residues 9-16) is packed against both the sheet and the helix with the two conserved disulfide bonds tethering the N-terminal/N-loop region to the beta-sheet. The average backbone and heavy atom rmsd values of the 20 structures (residues 7-66) are 0.52 and 1.13 A, respectively. A linear peptide corresponding to the N-terminal region of CCR3 binds to eotaxin-2, inducing concentration-dependent chemical shift changes or line broadening of many residues. The distribution of these residues suggests that the peptide binds into an extended groove located at the interface between the N-loop and the beta2-beta3 hairpin. The receptor peptide may also interact with the N-terminus of the chemokine and part of the alpha-helix. Comparison of the eotaxin-2 structure with those of related chemokines indicates several structural features that may contribute to receptor specificity.
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<StructureSection load='1eih' size='340' side='right'caption='[[1eih]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1eih]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EIH FirstGlance]. <br>
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1EIH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eih OCA], [https://pdbe.org/1eih PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eih RCSB], [https://www.ebi.ac.uk/pdbsum/1eih PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eih ProSAT]</span></td></tr>
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==Reference==
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</table>
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NMR solution structure and receptor peptide binding of the CC chemokine eotaxin-2., Mayer KL, Stone MJ, Biochemistry. 2000 Jul 25;39(29):8382-95. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10913244 10913244]
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== Function ==
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[https://www.uniprot.org/uniprot/CCL24_HUMAN CCL24_HUMAN] Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/1eih_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eih ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Mayer, K L.]]
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[[Category: Mayer KL]]
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[[Category: Stone, M J.]]
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[[Category: Stone MJ]]
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[[Category: chemokine]]
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[[Category: chemotactic cytokine]]
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[[Category: eosinophil chemoattractant]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:28:06 2008''
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Current revision

SOLUTION STRUCTURE OF THE HUMAN CHEMOKINE EOTAXIN-2

PDB ID 1eih

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