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3lwv

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[[Image:3lwv.png|left|200px]]
 
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{{STRUCTURE_3lwv| PDB=3lwv | SCENE= }}
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==Structure of H/ACA RNP bound to a substrate RNA containing 2'-deoxyuridine==
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<StructureSection load='3lwv' size='340' side='right'caption='[[3lwv]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3lwv]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LWV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3LWV FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DU:2-DEOXYURIDINE-5-MONOPHOSPHATE'>DU</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3lwo|3lwo]], [[3lwp|3lwp]], [[3lwq|3lwq]], [[3lwr|3lwr]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3lwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lwv OCA], [http://pdbe.org/3lwv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3lwv RCSB], [http://www.ebi.ac.uk/pdbsum/3lwv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3lwv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/TRUB_PYRFU TRUB_PYRFU]] Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs (By similarity). [[http://www.uniprot.org/uniprot/RL7A_PYRFU RL7A_PYRFU]] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, box H/ACA, box C/D and box C'/D' sRNAs (By similarity). [[http://www.uniprot.org/uniprot/NOP10_PYRFU NOP10_PYRFU]] Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lw/3lwv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lwv ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Box H/ACA ribonucleoprotein protein particles catalyze the majority of pseudouridylation in functional RNA. Different from stand alone pseudouridine synthases, the RNP pseudouridine synthase comprises multiple protein subunits and an RNA subunit. Previous studies showed that each subunit, regardless its location, is sensitive to the step of subunit placement at the catalytic center and potentially to the reaction status of the substrate. Here we describe the impact of chemical substitutions of target uridine on enzyme activity and structure. We found that 3-methyluridine in place of uridine inhibited its isomerization while 2'-deoxyuridine or 4-thiouridine did not. Significantly, crystal structures of an archaeal box H/ACA RNP bound with the nonreactive and the two postreactive substrate analogues showed only subtle structural changes throughout the assembly except for a conserved tyrosine and a substrate anchoring loop of Cbf5. Our results suggest a potential role of these elements and the subunit that contacts them in substrate binding and product release.
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===Structure of H/ACA RNP bound to a substrate RNA containing 2'-deoxyuridine===
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Functional and Structural Impact of Target Uridine Substitutions on the H/ACA Ribonucleoprotein Particle Pseudouridine Synthase .,Zhou J, Liang B, Li H Biochemistry. 2010 Jul 2. PMID:20575532<ref>PMID:20575532</ref>
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{{ABSTRACT_PUBMED_20575532}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3lwv" style="background-color:#fffaf0;"></div>
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[[3lwv]] is a 5 chain structure of [[Ribosomal protein L7]] and [[TRNA pseudouridine synthase]] with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LWV OCA].
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==See Also==
==See Also==
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*[[Ribosomal protein L7|Ribosomal protein L7]]
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*[[Guide-independent Pseudouridine synthase|Guide-independent Pseudouridine synthase]]
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*[[TRNA pseudouridine synthase|TRNA pseudouridine synthase]]
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*[[Pseudouridine synthase 3D structures|Pseudouridine synthase 3D structures]]
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*[[Ribosome biogenesis protein|Ribosome biogenesis protein]]
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==Reference==
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== References ==
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<ref group="xtra">PMID:020575532</ref><references group="xtra"/>
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<references/>
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[[Category: Pyrococcus furiosus]]
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__TOC__
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[[Category: Li, H.]]
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</StructureSection>
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[[Category: Liang, B.]]
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[[Category: Atcc 43587]]
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[[Category: Lv, C.]]
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[[Category: Large Structures]]
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[[Category: Yang, W.]]
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[[Category: Li, H]]
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[[Category: Zhou, J.]]
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[[Category: Liang, B]]
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[[Category: Lv, C]]
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[[Category: Yang, W]]
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[[Category: Zhou, J]]
[[Category: H/aca pseudouridine synthase]]
[[Category: H/aca pseudouridine synthase]]
[[Category: Isomerase]]
[[Category: Isomerase]]

Current revision

Structure of H/ACA RNP bound to a substrate RNA containing 2'-deoxyuridine

PDB ID 3lwv

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