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1elt

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[[Image:1elt.gif|left|200px]]<br /><applet load="1elt" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1elt, resolution 1.61&Aring;" />
 
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'''STRUCTURE OF NATIVE PANCREATIC ELASTASE FROM NORTH ATLANTIC SALMON AT 1.61 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==STRUCTURE OF NATIVE PANCREATIC ELASTASE FROM NORTH ATLANTIC SALMON AT 1.61 ANGSTROMS RESOLUTION==
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The crystal structure of native salmon pancreatic elastase (SPE) has been solved by molecular-replacement methods, and refined by conventional conjugate-gradient methods and simulated-annealing techniques. The final R value is 17.2% for 21 389 reflections between 8.0 and 1.61 A, and the corresponding free R value is 23.9%. The overall tertiary structure of SPE is remarkably similar to that of porcine pancreatic elastase I (PPE), to which it shows about 67% sequence identity. The primary structure of SPE is determined from the electron-density maps, and only about 15 side chains are somewhat uncertain. Interesting differences between SPE and PPE, are one sequence deletion assigned to position 186, the residue 192 at the entrance of the specificity pocket is substituted from a Gln in PPE to Asn in SPE, and one of the calcium ligands is different. Furthermore, electron density is missing in SPE for the last three residues of the C-terminal helix. A comparison of the present amino-acid sequence of SPE with other sequences available indicates that SPE belongs to the class 1 pancreatic elastases.
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<StructureSection load='1elt' size='340' side='right'caption='[[1elt]], [[Resolution|resolution]] 1.61&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1elt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmo_salar Salmo salar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ELT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.61&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1elt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1elt OCA], [https://pdbe.org/1elt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1elt RCSB], [https://www.ebi.ac.uk/pdbsum/1elt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1elt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ELA1_SALSA ELA1_SALSA] Acts upon elastin.<ref>PMID:9628006</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/el/1elt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1elt ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1ELT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmo_salar Salmo salar] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELT OCA].
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*[[Elastase 3D structures|Elastase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure of native pancreatic elastase from North Atlantic salmon at 1.61 A resolution., Berglund GI, Willassen NP, Hordvik A, Smalas AO, Acta Crystallogr D Biol Crystallogr. 1995 Nov 1;51(Pt 6):925-37. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299762 15299762]
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__TOC__
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[[Category: Pancreatic elastase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Salmo salar]]
[[Category: Salmo salar]]
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[[Category: Single protein]]
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[[Category: Berglund GI]]
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[[Category: Berglund, G I.]]
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[[Category: Smalaas AO]]
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[[Category: Smalaas, A O.]]
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[[Category: CA]]
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[[Category: serine proteinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:29:06 2008''
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Current revision

STRUCTURE OF NATIVE PANCREATIC ELASTASE FROM NORTH ATLANTIC SALMON AT 1.61 ANGSTROMS RESOLUTION

PDB ID 1elt

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