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3kuy

From Proteopedia

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[[Image:3kuy.png|left|200px]]
 
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{{STRUCTURE_3kuy| PDB=3kuy | SCENE= }}
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==DNA Stretching in the Nucleosome Facilitates Alkylation by an Intercalating Antitumor Agent==
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<StructureSection load='3kuy' size='340' side='right'caption='[[3kuy]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3kuy]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KUY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KUY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATV:2-[(2R)-OXIRAN-2-YLMETHYL]-1H-BENZO[DE]ISOQUINOLINE-1,3(2H)-DIONE'>ATV</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kuy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kuy OCA], [https://pdbe.org/3kuy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kuy RCSB], [https://www.ebi.ac.uk/pdbsum/3kuy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kuy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/H32_XENLA H32_XENLA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ku/3kuy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kuy ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DNA stretching in the nucleosome core can cause dramatic structural distortions, which may influence compaction and factor recognition in chromatin. We find that the base pair unstacking arising from stretching-induced extreme minor groove kinking near the nucleosome centre creates a hot spot for intercalation and alkylation by a novel anticancer compound. This may have far reaching implications for how chromatin structure can influence binding of intercalator species and indicates potential for the development of site selective DNA-binding agents that target unique conformational features of the nucleosome.
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===DNA Stretching in the Nucleosome Facilitates Alkylation by an Intercalating Antitumor Agent===
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DNA stretching in the nucleosome facilitates alkylation by an intercalating antitumour agent.,Davey GE, Wu B, Dong Y, Surana U, Davey CA Nucleic Acids Res. 2010 Apr;38(6):2081-8. Epub 2009 Dec 21. PMID:20026584<ref>PMID:20026584</ref>
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{{ABSTRACT_PUBMED_20026584}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3kuy" style="background-color:#fffaf0;"></div>
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[[3kuy]] is a 10 chain structure of [[Histone]] with sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KUY OCA].
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==See Also==
==See Also==
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*[[Histone|Histone]]
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*[[Histone 3D structures|Histone 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020026584</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Xenopus laevis]]
[[Category: Xenopus laevis]]
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[[Category: Davey, C A.]]
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[[Category: Davey CA]]
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[[Category: Wu, B.]]
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[[Category: Wu B]]
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[[Category: Alkylation]]
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[[Category: Chromatin]]
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[[Category: Chromosomal protein]]
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[[Category: Dna stretching]]
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[[Category: Dna-binding]]
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[[Category: Intercalation]]
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[[Category: Methylation]]
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[[Category: Nucleosome]]
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[[Category: Nucleosome core]]
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[[Category: Nucleus]]
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[[Category: Structural protein-dna complex]]
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Current revision

DNA Stretching in the Nucleosome Facilitates Alkylation by an Intercalating Antitumor Agent

PDB ID 3kuy

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