1s3k

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[[Image:1s3k.png|left|200px]]
 
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{{STRUCTURE_1s3k| PDB=1s3k | SCENE= }}
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==Crystal Structure of a Humanized Fab (hu3S193) in Complex with the Lewis Y Tetrasaccharide==
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<StructureSection load='1s3k' size='340' side='right'caption='[[1s3k]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1s3k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S3K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S3K FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=PRD_900124:Lewis+Y+antigen,+alpha+anomer'>PRD_900124</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s3k OCA], [https://pdbe.org/1s3k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s3k RCSB], [https://www.ebi.ac.uk/pdbsum/1s3k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s3k ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s3/1s3k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s3k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Antibodies targeting human epithelial carcinomas bearing Lewis Y (Le(y)) carbohydrate antigens provide a striking illustration of convergent immune recognition. We report a 1.9A resolution crystal structure of the Fab of a humanized antibody (hu3S193) in complex with the Le(y) tetrasaccharide, Fuc(alpha 1--&gt;2)Gal(beta 1--&gt;4)[Fuc(alpha 1--&gt;3)]GlcNAc. Comparisons of the hu3S193 and BR96 antibodies bound to Le(y) tumor antigens revealed extremely similar mechanisms for recognition of the carbohydrate epitopes. Solvent plays a critical role in hu3S193 antibody binding to the Le(y) carbohydrate epitope. Specificity for Le(y) is maintained because a conserved pocket accepts an N-acetyl group of the core Gal(beta 1--&gt;4)GlcNAc disaccharide. Closely related blood-group determinants (Le(a) and Le(b)) cannot enter the specificity pocket, making the Le(y) antibodies promising candidates for immunotherapy of epithelial cancer.
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===Crystal Structure of a Humanized Fab (hu3S193) in Complex with the Lewis Y Tetrasaccharide===
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Structural convergence of antibody binding of carbohydrate determinants in Lewis Y tumor antigens.,Ramsland PA, Farrugia W, Bradford TM, Mark Hogarth P, Scott AM J Mol Biol. 2004 Jul 16;340(4):809-18. PMID:15223322<ref>PMID:15223322</ref>
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{{ABSTRACT_PUBMED_15223322}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1s3k" style="background-color:#fffaf0;"></div>
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[[1s3k]] is a 2 chain structure of [[Monoclonal Antibody]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens,_mus_musculus Homo sapiens, mus musculus] and [http://en.wikipedia.org/wiki/Mus_musculus,_homo_sapiens Mus musculus, homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S3K OCA].
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==See Also==
==See Also==
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*[[Monoclonal Antibody|Monoclonal Antibody]]
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:015223322</ref><references group="xtra"/>
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__TOC__
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[[Category: Homo sapiens, mus musculus]]
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</StructureSection>
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[[Category: Mus musculus, homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Bradford, T M.]]
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[[Category: Mus musculus]]
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[[Category: Farrugia, W.]]
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[[Category: Bradford TM]]
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[[Category: Hogarth, P M.]]
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[[Category: Farrugia W]]
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[[Category: Ramsland, P A.]]
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[[Category: Hogarth PM]]
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[[Category: Scott, A M.]]
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[[Category: Ramsland PA]]
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[[Category: Antigen binding fragment]]
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[[Category: Scott AM]]
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[[Category: Beta barrel]]
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[[Category: Humanized antibody]]
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[[Category: Immune system]]
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[[Category: Immunoglobulin fold]]
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Current revision

Crystal Structure of a Humanized Fab (hu3S193) in Complex with the Lewis Y Tetrasaccharide

PDB ID 1s3k

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