3l0x

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[[Image:3l0x.png|left|200px]]
 
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{{STRUCTURE_3l0x| PDB=3l0x | SCENE= }}
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==Structure of split yeast PCNA==
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<StructureSection load='3l0x' size='340' side='right'caption='[[3l0x]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3l0x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L0X FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l0x OCA], [https://pdbe.org/3l0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l0x RCSB], [https://www.ebi.ac.uk/pdbsum/3l0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l0x ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PCNA_YEAST PCNA_YEAST] This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.<ref>PMID:11545742</ref> <ref>PMID:12226657</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l0/3l0x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3l0x ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DNA synthesis by classical polymerases can be blocked by many lesions. These blocks are overcome by translesion synthesis, whereby the stalled classical, replicative polymerase is replaced by a nonclassical polymerase. In eukaryotes this polymerase exchange requires proliferating cell nuclear antigen (PCNA) monoubiquitination. To better understand the polymerase exchange, we developed a means of producing monoubiquitinated PCNA, by splitting the protein into two self-assembling polypeptides. We determined the X-ray crystal structure of monoubiquitinated PCNA and found that the ubiquitin moieties are located on the back face of PCNA and interact with it through their canonical hydrophobic surface. Moreover, the attachment of ubiquitin does not change PCNA's conformation. We propose that PCNA ubiquitination facilitates nonclassical polymerase recruitment to the back of PCNA by forming a new binding surface for nonclassical polymerases, consistent with a 'tool belt' model of the polymerase exchange.
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===Structure of split yeast PCNA===
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Structure of monoubiquitinated PCNA and implications for translesion synthesis and DNA polymerase exchange.,Freudenthal BD, Gakhar L, Ramaswamy S, Washington MT Nat Struct Mol Biol. 2010 Apr;17(4):479-84. Epub 2010 Mar 21. PMID:20305653<ref>PMID:20305653</ref>
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{{ABSTRACT_PUBMED_20305653}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3l0x" style="background-color:#fffaf0;"></div>
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[[3l0x]] is a 2 chain structure of [[Proliferating Cell Nuclear Antigen]] with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L0X OCA].
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==See Also==
==See Also==
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*[[Proliferating Cell Nuclear Antigen|Proliferating Cell Nuclear Antigen]]
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*[[Proliferating cell nuclear antigen 3D structures|Proliferating cell nuclear antigen 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020305653</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Freudenthal, B D.]]
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[[Category: Freudenthal BD]]
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[[Category: Gakhar, L.]]
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[[Category: Gakhar L]]
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[[Category: Ramaswamy, S.]]
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[[Category: Ramaswamy S]]
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[[Category: Washington, M T.]]
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[[Category: Washington MT]]
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[[Category: Dna damage]]
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[[Category: Dna repair]]
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[[Category: Dna replication]]
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[[Category: Dna-binding]]
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[[Category: Isopeptide bond]]
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[[Category: Nucleus]]
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[[Category: Replication]]
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Current revision

Structure of split yeast PCNA

PDB ID 3l0x

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