1ew3

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[[Image:1ew3.gif|left|200px]]<br /><applet load="1ew3" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ew3, resolution 2.3&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE MAJOR HORSE ALLERGEN EQU C 1'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE MAJOR HORSE ALLERGEN EQU C 1==
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The three-dimensional structure of the major horse allergen Equ c 1 has been determined at 2.3 A resolution by x-ray crystallography. Equ c 1 displays the typical fold of lipocalins, a beta-barrel flanked by a C-terminal alpha-helix. The space between the two beta-sheets of the barrel defines an internal cavity that could serve, as in other lipocalins, for the binding and transport of small hydrophobic ligands. Equ c 1 crystallizes in a novel dimeric form, which is distinct from that observed in other lipocalin dimers and corresponds to the functional form of the allergen. Binding studies of point mutants of the allergen with specific monoclonal antibodies raised in mouse and IgE serum from horse allergic patients allowed to identify putative B cell antigenic determinants. In addition, total inhibition of IgE serum recognition by a single specific monoclonal antibody revealed the restricted nature of the IgE binding target on the molecular surface of Equ c 1.
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<StructureSection load='1ew3' size='340' side='right'caption='[[1ew3]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ew3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EW3 FirstGlance]. <br>
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1EW3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EW3 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ew3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ew3 OCA], [https://pdbe.org/1ew3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ew3 RCSB], [https://www.ebi.ac.uk/pdbsum/1ew3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ew3 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of the allergen Equ c 1. A dimeric lipocalin with restricted IgE-reactive epitopes., Lascombe MB, Gregoire C, Poncet P, Tavares GA, Rosinski-Chupin I, Rabillon J, Goubran-Botros H, Mazie JC, David B, Alzari PM, J Biol Chem. 2000 Jul 14;275(28):21572-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10787420 10787420]
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== Function ==
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[https://www.uniprot.org/uniprot/ALL1_HORSE ALL1_HORSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/1ew3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ew3 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Equus caballus]]
[[Category: Equus caballus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Alzari, P M.]]
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[[Category: Alzari PM]]
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[[Category: David, B.]]
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[[Category: David B]]
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[[Category: Goubran-Botros, H.]]
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[[Category: Goubran-Botros H]]
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[[Category: Gregoire, C.]]
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[[Category: Gregoire C]]
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[[Category: Lascombe, M B.]]
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[[Category: Lascombe MB]]
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[[Category: Mazie, J C.]]
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[[Category: Mazie JC]]
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[[Category: Poncet, P.]]
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[[Category: Poncet P]]
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[[Category: Rabillon, J.]]
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[[Category: Rabillon J]]
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[[Category: Rosinski-Chupin, I.]]
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[[Category: Rosinski-Chupin I]]
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[[Category: Tavares, G A.]]
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[[Category: Tavares GA]]
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[[Category: beta barrel]]
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[[Category: lipocalin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:32:07 2008''
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Current revision

CRYSTAL STRUCTURE OF THE MAJOR HORSE ALLERGEN EQU C 1

PDB ID 1ew3

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