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1eww

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[[Image:1eww.jpg|left|200px]]<br /><applet load="1eww" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eww" />
 
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'''SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEGREES CELSIUS'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEGREES CELSIUS==
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Insect antifreeze proteins (AFP) are considerably more active at inhibiting ice crystal growth than AFP from fish or plants. Several insect AFPs, also known as thermal hysteresis proteins, have been cloned and expressed. Their maximum activity is 3-4 times that of fish AFPs and they are 10-100 times more effective at micromolar concentrations. Here we report the solution structure of spruce budworm (Choristoneura fumiferana) AFP and characterize its ice-binding properties. The 9-kDa AFP is a beta-helix with a triangular cross-section and rectangular sides that form stacked parallel beta-sheets; a fold which is distinct from the three known fish AFP structures. The ice-binding side contains 9 of the 14 surface-accessible threonines organized in a regular array of TXT motifs that match the ice lattice on both prism and basal planes. In support of this model, ice crystal morphology and ice-etching experiments are consistent with AFP binding to both of these planes and thus may explain the greater activity of the spruce budworm antifreeze.
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<StructureSection load='1eww' size='340' side='right'caption='[[1eww]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. The December 2009 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EWW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eww OCA], [https://pdbe.org/1eww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eww RCSB], [https://www.ebi.ac.uk/pdbsum/1eww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eww ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9GTP0_CHOFU Q9GTP0_CHOFU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/1eww_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eww ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EWW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA].
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*[[Antifreeze protein 3D structures|Antifreeze protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Beta-helix structure and ice-binding properties of a hyperactive antifreeze protein from an insect., Graether SP, Kuiper MJ, Gagne SM, Walker VK, Jia Z, Sykes BD, Davies PL, Nature. 2000 Jul 20;406(6793):325-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10917537 10917537]
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[[Category: Antifreeze Proteins]]
[[Category: Choristoneura fumiferana]]
[[Category: Choristoneura fumiferana]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Davies, P L.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Gagne, S M.]]
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[[Category: Davies PL]]
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[[Category: Graether, S P.]]
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[[Category: Gagne SM]]
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[[Category: Jia, Z.]]
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[[Category: Graether SP]]
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[[Category: Kuiper, M J.]]
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[[Category: Jia Z]]
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[[Category: Sykes, B D.]]
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[[Category: Kuiper MJ]]
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[[Category: Walker, V K.]]
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[[Category: Sykes BD]]
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[[Category: antifreeze protein]]
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[[Category: Walker VK]]
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[[Category: beta-helix]]
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[[Category: ice]]
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[[Category: insect]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:32:21 2008''
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Current revision

SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEGREES CELSIUS

PDB ID 1eww

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