1eyq

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[[Image:1eyq.jpg|left|200px]]<br /><applet load="1eyq" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eyq, resolution 1.85&Aring;" />
 
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'''CHALCONE ISOMERASE AND NARINGENIN'''<br />
 
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==Overview==
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==Chalcone isomerase and naringenin==
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Chalcone isomerase (CHI) catalyzes the intramolecular cyclization of chalcone synthesized by chalcone synthase (CHS) into (2S)-naringenin, an essential compound in the biosynthesis of anthocyanin pigments, inducers of Rhizobium nodulation genes, and antimicrobial phytoalexins. The 1.85 A resolution crystal structure of alfalfa CHI in complex with (2S)-naringenin reveals a novel open-faced beta-sandwich fold. Currently, proteins with homologous primary sequences are found only in higher plants. The topology of the active site cleft defines the stereochemistry of the cyclization reaction. The structure and mutational analysis suggest a mechanism in which shape complementarity of the binding cleft locks the substrate into a constrained conformation that allows the reaction to proceed with a second-order rate constant approaching the diffusion controlled limit. This structure raises questions about the evolutionary history of this structurally unique plant enzyme.
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<StructureSection load='1eyq' size='340' side='right'caption='[[1eyq]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1eyq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EYQ FirstGlance]. <br>
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1EYQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=NAR:'>NAR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chalcone_isomerase Chalcone isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.6 5.5.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYQ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAR:NARINGENIN'>NAR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eyq OCA], [https://pdbe.org/1eyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eyq RCSB], [https://www.ebi.ac.uk/pdbsum/1eyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eyq ProSAT]</span></td></tr>
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Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase., Jez JM, Bowman ME, Dixon RA, Noel JP, Nat Struct Biol. 2000 Sep;7(9):786-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10966651 10966651]
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</table>
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[[Category: Chalcone isomerase]]
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== Function ==
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[https://www.uniprot.org/uniprot/CFI1_MEDSA CFI1_MEDSA] Catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. Responsible for the isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone) into naringenin.<ref>PMID:10966651</ref> <ref>PMID:11955065</ref> <ref>PMID:11698411</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ey/1eyq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eyq ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Medicago sativa]]
[[Category: Medicago sativa]]
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[[Category: Single protein]]
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[[Category: Bowman ME]]
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[[Category: Bowman, M E.]]
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[[Category: Dixon RA]]
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[[Category: Dixon, R A.]]
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[[Category: Jez JM]]
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[[Category: Jez, J M.]]
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[[Category: Noel JP]]
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[[Category: Noel, J P.]]
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[[Category: NAR]]
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[[Category: SO4]]
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[[Category: chalcone isomerase]]
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[[Category: flavonoid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:33:01 2008''
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Current revision

Chalcone isomerase and naringenin

PDB ID 1eyq

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