2rf4

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[[Image:2rf4.png|left|200px]]
 
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{{STRUCTURE_2rf4| PDB=2rf4 | SCENE= }}
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==Crystal structure of the RNA Polymerase I subcomplex A14/43==
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<StructureSection load='2rf4' size='340' side='right'caption='[[2rf4]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2rf4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RF4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rf4 OCA], [https://pdbe.org/2rf4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rf4 RCSB], [https://www.ebi.ac.uk/pdbsum/2rf4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rf4 ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rf/2rf4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rf4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Synthesis of ribosomal RNA (rRNA) by RNA polymerase (Pol) I is the first step in ribosome biogenesis and a regulatory switch in eukaryotic cell growth. Here we report the 12 A cryo-electron microscopic structure for the complete 14-subunit yeast Pol I, a homology model for the core enzyme, and the crystal structure of the subcomplex A14/43. In the resulting hybrid structure of Pol I, A14/43, the clamp, and the dock domain contribute to a unique surface interacting with promoter-specific initiation factors. The Pol I-specific subunits A49 and A34.5 form a heterodimer near the enzyme funnel that acts as a built-in elongation factor and is related to the Pol II-associated factor TFIIF. In contrast to Pol II, Pol I has a strong intrinsic 3'-RNA cleavage activity, which requires the C-terminal domain of subunit A12.2 and, apparently, enables ribosomal RNA proofreading and 3'-end trimming.
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===Crystal structure of the RNA Polymerase I subcomplex A14/43===
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Functional architecture of RNA polymerase I.,Kuhn CD, Geiger SR, Baumli S, Gartmann M, Gerber J, Jennebach S, Mielke T, Tschochner H, Beckmann R, Cramer P Cell. 2007 Dec 28;131(7):1260-72. PMID:18160037<ref>PMID:18160037</ref>
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{{ABSTRACT_PUBMED_18160037}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2rf4" style="background-color:#fffaf0;"></div>
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[[2rf4]] is a 6 chain structure of [[RNA polymerase]] with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RF4 OCA].
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==See Also==
==See Also==
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*[[RNA polymerase|RNA polymerase]]
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:018160037</ref><references group="xtra"/>
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__TOC__
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[[Category: DNA-directed RNA polymerase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Cramer, P.]]
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[[Category: Cramer P]]
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[[Category: Geiger, S R.]]
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[[Category: Geiger SR]]
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[[Category: Kuhn, C D.]]
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[[Category: Kuhn CD]]
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[[Category: Ddrp]]
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[[Category: Dna-binding]]
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[[Category: Dna-directed rna polymerase]]
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[[Category: Nuclear protein]]
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[[Category: Nucleolar protein]]
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[[Category: Nucleus]]
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[[Category: Phosphorylation]]
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[[Category: Pol i]]
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[[Category: Poli]]
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[[Category: Ribosome biogenesis]]
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[[Category: Rna polymerase i]]
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[[Category: Rpb4/7]]
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[[Category: Rpoli]]
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[[Category: Transcription]]
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[[Category: Transferase]]
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[[Category: Transferase dna/rna]]
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Current revision

Crystal structure of the RNA Polymerase I subcomplex A14/43

PDB ID 2rf4

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