2ofd

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2ofd" [edit=sysop:move=sysop])
Current revision (08:23, 30 October 2024) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2ofd.png|left|200px]]
 
-
{{STRUCTURE_2ofd| PDB=2ofd | SCENE= }}
+
==The Crystal Structure of Sclerotium rolfsii lectin in complex with N-acetyl-D-galactosamine==
 +
<StructureSection load='2ofd' size='340' side='right'caption='[[2ofd]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2ofd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Athelia_rolfsii Athelia rolfsii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OFD FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ofd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofd OCA], [https://pdbe.org/2ofd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ofd RCSB], [https://www.ebi.ac.uk/pdbsum/2ofd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ofd ProSAT]</span></td></tr>
 +
</table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/2ofd_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ofd ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The crystal structure of a novel fungal lectin from Sclerotium rolfsii (SRL) in its free form and in complex with N-acetyl-d-galactosamine (GalNAc) and N-acetyl- d -glucosamine (GlcNAc) has been determined at 1.1 A, 2.0 A, and 1.7 A resolution, respectively. The protein structure is composed of two beta-sheets, which consist of four and six beta-strands, connected by two alpha-helices. Sequence and structural comparisons reveal that SRL is the third member of a newly identified family of fungal lectins, which includes lectins from Agaricus bisporus and Xerocomus chrysenteron that share a high degree of structural similarity and carbohydrate specificity. The data for the free SRL are the highest resolution data for any protein of this family. The crystal structures of the SRL in complex with two carbohydrates, GalNAc and GlcNAc, which differ only in the configuration of a single epimeric hydroxyl group, provide the structural basis for its carbohydrate specificity. SRL has two distinct carbohydrate-binding sites, a primary and a secondary. GalNAc binds at the primary site, whereas GlcNAc binds only at the secondary site. Thus, SRL has the ability to recognize and probably bind at the same time two different carbohydrate structures. Structural comparison to Agaricus bisporus lectin-carbohydrate complexes reveals that the primary site is also able to bind the Thomsen-Friedenreich antigen (Galbeta1--&gt;3GalNAc-alpha- glycan structures) whereas the secondary site cannot. The features of the molecular recognition at the two sites are described in detail.
-
===The Crystal Structure of Sclerotium rolfsii lectin in complex with N-acetyl-D-galactosamine===
+
Structural basis for the carbohydrate recognition of the Sclerotium rolfsii lectin.,Leonidas DD, Swamy BM, Hatzopoulos GN, Gonchigar SJ, Chachadi VB, Inamdar SR, Zographos SE, Oikonomakos NG J Mol Biol. 2007 May 11;368(4):1145-61. Epub 2007 Mar 7. PMID:17391699<ref>PMID:17391699</ref>
-
{{ABSTRACT_PUBMED_17391699}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 2ofd" style="background-color:#fffaf0;"></div>
-
[[2ofd]] is a 2 chain structure of [[Ribosomal protein L13]] with sequence from [http://en.wikipedia.org/wiki/Athelia_rolfsii Athelia rolfsii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFD OCA].
+
== References ==
-
 
+
<references/>
-
==See Also==
+
__TOC__
-
*[[Ribosomal protein L13|Ribosomal protein L13]]
+
</StructureSection>
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:017391699</ref><references group="xtra"/>
+
[[Category: Athelia rolfsii]]
[[Category: Athelia rolfsii]]
-
[[Category: Leonidas, D D.]]
+
[[Category: Large Structures]]
-
[[Category: Oikonomakos, N G.]]
+
[[Category: Leonidas DD]]
-
[[Category: Zographos, S E.]]
+
[[Category: Oikonomakos NG]]
-
[[Category: Dual specificity]]
+
[[Category: Zographos SE]]
-
[[Category: Lectin]]
+
-
[[Category: Sugar binding protein]]
+

Current revision

The Crystal Structure of Sclerotium rolfsii lectin in complex with N-acetyl-D-galactosamine

PDB ID 2ofd

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ofd"
Personal tools