1f4k

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[[Image:1f4k.gif|left|200px]]<br /><applet load="1f4k" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1f4k, resolution 2.5&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE REPLICATION TERMINATOR PROTEIN/B-SITE DNA COMPLEX'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE REPLICATION TERMINATOR PROTEIN/B-SITE DNA COMPLEX==
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The coordinated termination of DNA replication is an important step in the life cycle of bacteria with circular chromosomes, but has only been defined at a molecular level in two systems to date. Here we report the structure of an engineered replication terminator protein (RTP) of Bacillus subtilis in complex with a 21 base pair DNA by X-ray crystallography at 2.5 A resolution. We also use NMR spectroscopic titration techniques. This work reveals a novel DNA interaction involving a dimeric 'winged helix' domain protein that differs from predictions. While the two recognition helices of RTP are in close contact with the B-form DNA major grooves, the 'wings' and N-termini of RTP do not form intimate contacts with the DNA. This structure provides insight into the molecular basis of polar replication fork arrest based on a model of cooperative binding and differential binding affinities of RTP to the two adjacent binding sites in the complete terminator.
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<StructureSection load='1f4k' size='340' side='right'caption='[[1f4k]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1f4k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F4K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F4K FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f4k OCA], [https://pdbe.org/1f4k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f4k RCSB], [https://www.ebi.ac.uk/pdbsum/1f4k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f4k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RTP_BACSU RTP_BACSU] Plays a role in DNA replication and termination (fork arrest mechanism). Two dimers of rtp bind to the two inverted repeat regions (IRI and IRII) present in the termination site. The binding of each dimer is centered on an 8 bp direct repeat.
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==About this Structure==
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==See Also==
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1F4K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F4K OCA].
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*[[Replication Termination Protein|Replication Termination Protein]]
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*[[Tomato aspermy virus protein 2b Suppression of RNA Silencing|Tomato aspermy virus protein 2b Suppression of RNA Silencing]]
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==Reference==
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__TOC__
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Structure of the RTP-DNA complex and the mechanism of polar replication fork arrest., Wilce JA, Vivian JP, Hastings AF, Otting G, Folmer RH, Duggin IG, Wake RG, Wilce MC, Nat Struct Biol. 2001 Mar;8(3):206-10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11224562 11224562]
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Duggin, I G.]]
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[[Category: Duggin IG]]
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[[Category: Folmer, R H.A.]]
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[[Category: Folmer RHA]]
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[[Category: Hastings, A F.]]
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[[Category: Hastings AF]]
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[[Category: Otting, G.]]
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[[Category: Otting G]]
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[[Category: Vivian, J P.]]
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[[Category: Vivian JP]]
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[[Category: Wake, R G.]]
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[[Category: Wake RG]]
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[[Category: Wilce, J A.]]
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[[Category: Wilce JA]]
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[[Category: Wilce, M C.J.]]
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[[Category: Wilce MCJ]]
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[[Category: fork arrest mechanism]]
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[[Category: replication and termination]]
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[[Category: winged-helix protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:34:49 2008''
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Current revision

CRYSTAL STRUCTURE OF THE REPLICATION TERMINATOR PROTEIN/B-SITE DNA COMPLEX

PDB ID 1f4k

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