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3hj6
From Proteopedia
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| - | [[Image:3hj6.png|left|200px]] | ||
| - | + | ==Structure of Halothermothrix orenii fructokinase (FRK)== | |
| + | <StructureSection load='3hj6' size='340' side='right'caption='[[3hj6]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3hj6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Haloh Haloh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HJ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HJ6 FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Fructokinase Fructokinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.4 2.7.1.4] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hj6 OCA], [https://pdbe.org/3hj6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hj6 RCSB], [https://www.ebi.ac.uk/pdbsum/3hj6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hj6 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/3hj6_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hj6 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Fructokinase (FRK; EC 2.7.1.4) catalyzes the phosphorylation of d-fructose to d-fructose 6-phosphate (F6P). This irreversible and near rate-limiting step is a central and regulatory process in plants and bacteria, which channels fructose into a metabolically active state for glycolysis. Towards understanding the mechanism of FRK, here we report the crystal structure of a FRK homolog from a thermohalophilic bacterium Halothermothrixorenii (Hore_18220 in sequence databases). The structure of the Hore_18220 protein reveals a catalytic domain with a Rossmann-like fold and a beta-sheet "lid" for dimerization. Based on comparison of Hore_18220 to structures of related proteins, we propose its mechanism of action, in which the lid serves to regulate access to the substrate binding sites. Close relationship of Hore_18220 and plant FRK enzymes allows us to propose a model for the structure and function of FRKs. | ||
| - | + | Crystal structure of a fructokinase homolog from Halothermothrix orenii.,Chua TK, Seetharaman J, Kasprzak JM, Ng C, Patel BK, Love C, Bujnicki JM, Sivaraman J J Struct Biol. 2010 Sep;171(3):397-401. Epub 2010 May 21. PMID:20493950<ref>PMID:20493950</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 3hj6" style="background-color:#fffaf0;"></div> | |
| - | + | ||
==See Also== | ==See Also== | ||
*[[Fructokinase|Fructokinase]] | *[[Fructokinase|Fructokinase]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Fructokinase]] | [[Category: Fructokinase]] | ||
| - | [[Category: | + | [[Category: Haloh]] |
| - | [[Category: Bujnicki, J M | + | [[Category: Large Structures]] |
| - | [[Category: Chua, T K | + | [[Category: Bujnicki, J M]] |
| - | [[Category: Kasprzak, J M | + | [[Category: Chua, T K]] |
| - | [[Category: Love, C | + | [[Category: Kasprzak, J M]] |
| - | [[Category: Ng, C | + | [[Category: Love, C]] |
| - | [[Category: Patel, B K | + | [[Category: Ng, C]] |
| - | [[Category: Seetharaman, J | + | [[Category: Patel, B K]] |
| - | [[Category: Sivaraman, J | + | [[Category: Seetharaman, J]] |
| + | [[Category: Sivaraman, J]] | ||
[[Category: Carbohydrate metabolism]] | [[Category: Carbohydrate metabolism]] | ||
| - | [[Category: Fructokinase]] | ||
[[Category: Fructose]] | [[Category: Fructose]] | ||
[[Category: Kinase]] | [[Category: Kinase]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Current revision
Structure of Halothermothrix orenii fructokinase (FRK)
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Categories: Fructokinase | Haloh | Large Structures | Bujnicki, J M | Chua, T K | Kasprzak, J M | Love, C | Ng, C | Patel, B K | Seetharaman, J | Sivaraman, J | Carbohydrate metabolism | Fructose | Kinase | Transferase
