1f89

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[[Image:1f89.gif|left|200px]]<br /><applet load="1f89" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1f89, resolution 2.4&Aring;" />
 
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'''Crystal structure of Saccharomyces cerevisiae Nit3, a member of branch 10 of the nitrilase superfamily'''<br />
 
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==Overview==
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==Crystal structure of Saccharomyces cerevisiae Nit3, a member of branch 10 of the nitrilase superfamily==
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The crystal structure of a yeast hypothetical protein with sequence similarity to CN hydrolases has been determined to 2.4 A resolution by the multiwavelength anomalous dispersion (MAD) method. The protein folds as a four-layer alphabetabetaalpha sandwich and exists as a dimer in the crystal and in solution. It was selected in a structural genomics project as representative of CN hydrolases at a time when no structures had been determined for members of this family. Structures for two other members of the family have since been reported and the three proteins have similar topology and dimerization modes, which are distinct from those of other alphabetabetaalpha proteins whose structures are known. The dimers form an unusual eight-layer alphabetabetaalpha:alphabetabetaalpha structure. Although the precise enzymatic reactions catalyzed by the yeast protein are not known, considerable information about the active site may be deduced from conserved sequence motifs, comparative biochemical information, and comparison with known structures of hydrolase active sites. As with serine hydrolases, the active-site nucleophile (cysteine in this case) is positioned on a nucleophile elbow.
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<StructureSection load='1f89' size='340' side='right'caption='[[1f89]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1f89]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F89 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F89 FirstGlance]. <br>
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1F89 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F89 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f89 OCA], [https://pdbe.org/1f89 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f89 RCSB], [https://www.ebi.ac.uk/pdbsum/1f89 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f89 ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1f89 TOPSAN]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of a putative CN hydrolase from yeast., Kumaran D, Eswaramoorthy S, Gerchman SE, Kycia H, Studier FW, Swaminathan S, Proteins. 2003 Aug 1;52(2):283-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12833551 12833551]
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== Function ==
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[https://www.uniprot.org/uniprot/NIT3_YEAST NIT3_YEAST]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f8/1f89_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f89 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Burley SK]]
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[[Category: Burley, S K.]]
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[[Category: Eswaramoorthy S]]
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[[Category: Eswaramoorthy, S.]]
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[[Category: Kumaran D]]
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[[Category: Kumaran, D.]]
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[[Category: Studier FW]]
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[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
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[[Category: Swaminathan S]]
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[[Category: Studier, F W.]]
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[[Category: Swaminathan, S.]]
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[[Category: dimer]]
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[[Category: four layer sandwich]]
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[[Category: new york structural genomix research consortium]]
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[[Category: nitrilase]]
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[[Category: nysgxrc]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomics]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:35:55 2008''
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Current revision

Crystal structure of Saccharomyces cerevisiae Nit3, a member of branch 10 of the nitrilase superfamily

PDB ID 1f89

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