3two
From Proteopedia
(Difference between revisions)
| (4 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | [[Image:3two.png|left|200px]] | ||
| - | + | ==The crystal structure of CAD from Helicobacter pylori complexed with NADP(H)== | |
| + | <StructureSection load='3two' size='340' side='right'caption='[[3two]], [[Resolution|resolution]] 2.18Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3two]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_51 Helicobacter pylori 51]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TWO FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.18Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3two FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3two OCA], [https://pdbe.org/3two PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3two RCSB], [https://www.ebi.ac.uk/pdbsum/3two PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3two ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/D0ITF8_HELP1 D0ITF8_HELP1] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cinnamyl alcohol dehydrogenase is a zinc- and NADPH-dependent dehydrogenase catalyzing the reversible conversion of p-hydroxycinnamaldehydes to their corresponding hydroxycinnamyl alcohols. A CAD homolog from Helicobacter pylori (HpCAD) possesses broad substrate specificities like the plant CADs and additionally a dismutation activity converting benzaldehyde to benzyl alcohol and benzoic acid. We have determined the crystal structure of HpCAD complexed with NADP(H) at 2.18A resolution to get a better understanding of this class of CAD outside of plants. The structure of HpCAD is highly homologous to the sinapyl alcohol dehydrogenase and the plant CAD with well-conserved residues involved in catalysis and zinc binding. However, the NADP(H) binding mode of the HpCAD has been found to be significantly different from those of plant CADs. | ||
| - | + | Unusual NADPH conformation in the crystal structure of a cinnamyl alcohol dehydrogenase from Helicobacter pylori in complex with NADP(H) and substrate docking analysis.,Seo KH, Zhuang N, Chen C, Song JY, Kang HL, Rhee KH, Lee KH FEBS Lett. 2012 Feb 17;586(4):337-43. Epub 2012 Jan 21. PMID:22269576<ref>PMID:22269576</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 3two" style="background-color:#fffaf0;"></div> | |
| - | + | ||
==See Also== | ==See Also== | ||
| - | *[[Alcohol dehydrogenase|Alcohol dehydrogenase]] | + | *[[Alcohol dehydrogenase 3D structures|Alcohol dehydrogenase 3D structures]] |
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Helicobacter pylori 51]] |
| - | [[Category: Cong | + | [[Category: Large Structures]] |
| - | [[Category: Lee | + | [[Category: Cong C]] |
| - | [[Category: Seo | + | [[Category: Lee KH]] |
| - | [[Category: Zhuang | + | [[Category: Seo KH]] |
| - | + | [[Category: Zhuang NN]] | |
| - | + | ||
| - | + | ||
Current revision
The crystal structure of CAD from Helicobacter pylori complexed with NADP(H)
| |||||||||||
