1fft

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:14, 7 February 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1fft.gif|left|200px]]<br /><applet load="1fft" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1fft, resolution 3.5&Aring;" />
 
-
'''The structure of ubiquinol oxidase from Escherichia coli'''<br />
 
-
==Overview==
+
==The structure of ubiquinol oxidase from Escherichia coli==
-
Cell respiration is catalyzed by the heme-copper oxidase superfamily of enzymes, which comprises cytochrome c and ubiquinol oxidases. These membrane proteins utilize different electron donors through dissimilar access mechanisms. We report here the first structure of a ubiquinol oxidase, cytochrome bo3, from Escherichia coli. The overall structure of the enzyme is similar to those of cytochrome c oxidases; however, the membrane-spanning region of subunit I contains a cluster of polar residues exposed to the interior of the lipid bilayer that is not present in the cytochrome c oxidase. Mutagenesis studies on these residues strongly suggest that this region forms a quinone binding site. A sequence comparison of this region with known quinone binding sites in other membrane proteins shows remarkable similarities. In light of these findings we suggest specific roles for these polar residues in electron and proton transfer in ubiquinol oxidase.
+
<StructureSection load='1fft' size='340' side='right'caption='[[1fft]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1fft]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FFT FirstGlance]. <br>
-
1FFT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CU:'>CU</scene>, <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=HEO:'>HEO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FFT OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=HEO:HEME+O'>HEO</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fft OCA], [https://pdbe.org/1fft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fft RCSB], [https://www.ebi.ac.uk/pdbsum/1fft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fft ProSAT]</span></td></tr>
-
The structure of the ubiquinol oxidase from Escherichia coli and its ubiquinone binding site., Abramson J, Riistama S, Larsson G, Jasaitis A, Svensson-Ek M, Laakkonen L, Puustinen A, Iwata S, Wikstrom M, Nat Struct Biol. 2000 Oct;7(10):910-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11017202 11017202]
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CYOB_ECOLI CYOB_ECOLI] Cytochrome bo(3) ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron. Protons are probably pumped via D- and K- channels found in this subunit (PubMed:11017202).<ref>PMID:6308657</ref> <ref>PMID:19542282</ref> <ref>PMID:22843529</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ff/1fft_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fft ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
-
[[Category: Abramson, J.]]
+
[[Category: Abramson J]]
-
[[Category: Iwata, S.]]
+
[[Category: Iwata S]]
-
[[Category: Jasaitis, A.]]
+
[[Category: Jasaitis A]]
-
[[Category: Larsson, G.]]
+
[[Category: Larsson G]]
-
[[Category: Puustinen, A.]]
+
[[Category: Puustinen A]]
-
[[Category: Riistama, S.]]
+
[[Category: Riistama S]]
-
[[Category: Svensson-Ek, M.]]
+
[[Category: Svensson-Ek M]]
-
[[Category: Wikstrom, M.]]
+
[[Category: Wikstrom M]]
-
[[Category: CU]]
+
-
[[Category: HEM]]
+
-
[[Category: HEO]]
+
-
[[Category: cytochrome oxidase]]
+
-
[[Category: electron transport]]
+
-
[[Category: membrane protein]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:38:14 2008''
+

Current revision

The structure of ubiquinol oxidase from Escherichia coli

PDB ID 1fft

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fft"
Personal tools