3s0z

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[[Image:3s0z.png|left|200px]]
 
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{{STRUCTURE_3s0z| PDB=3s0z | SCENE= }}
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==Crystal structure of New Delhi Metallo-beta-lactamase (NDM-1)==
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<StructureSection load='3s0z' size='340' side='right'caption='[[3s0z]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3s0z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S0Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S0Z FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s0z OCA], [https://pdbe.org/3s0z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s0z RCSB], [https://www.ebi.ac.uk/pdbsum/3s0z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s0z ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/E5KIY2_ECOLX E5KIY2_ECOLX]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Gram-negative Enterobacteriaceae with resistance to carbapenem conferred by New Delhi metallo-beta-lactamase 1 (NDM-1) are a type of newly discovered antibioticresistant bacteria. The rapid pandemic spread of NDM-1 bacteria worldwide (spreading to India, Pakistan, Europe, America, and Chinese Taiwan) in less than 2 months characterizes these microbes as a potentially major global health problem. The drug resistance of NDM-1 bacteria is largely due to plasmids containing the blaNDM-1 gene shuttling through bacterial populations. The NDM-1 enzyme encoded by the blaNDM-1 gene hydrolyzes beta-lactam antibiotics, allowing the bacteria to escape the action of antibiotics. Although the biological functions and structural features of NDM-1 have been proposed according to results from functional and structural investigation of its homologues, the precise molecular characteristics and mechanism of action of NDM-1 have not been clarified. Here, we report the three-dimensional structure of NDM-1 with two catalytic zinc ions in its active site. Biological and mass spectroscopy results revealed that D-captopril can effectively inhibit the enzymatic activity of NDM-1 by binding to its active site with high binding affinity. The unique features concerning the primary sequence and structural conformation of the active site distinguish NDM-1 from other reported metallo-beta-lactamases (MBLs) and implicate its role in wide spectrum drug resistance. We also discuss the molecular mechanism of NDM-1 action and its essential role in the pandemic of drug-resistant NDM-1 bacteria. Our results will provide helpful information for future drug discovery targeting drug resistance caused by NDM-1 and related metallo-beta-lactamases.
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===Crystal structure of New Delhi Metallo-beta-lactamase (NDM-1)===
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A structural view of the antibiotic degradation enzyme NDM-1 from a superbug.,Guo Y, Wang J, Niu G, Shui W, Sun Y, Zhou H, Zhang Y, Yang C, Lou Z, Rao Z Protein Cell. 2011 May;2(5):384-94. Epub 2011 Jun 2. PMID:21637961<ref>PMID:21637961</ref>
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{{ABSTRACT_PUBMED_21637961}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3s0z" style="background-color:#fffaf0;"></div>
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[[3s0z]] is a 2 chain structure of [[Beta-lactamase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S0Z OCA].
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==See Also==
==See Also==
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*[[Beta-lactamase|Beta-lactamase]]
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*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021637961</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Guo, Y.]]
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[[Category: Large Structures]]
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[[Category: Lou, Z Y.]]
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[[Category: Guo Y]]
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[[Category: Niu, G J.]]
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[[Category: Lou ZY]]
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[[Category: Rao, Z H.]]
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[[Category: Niu GJ]]
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[[Category: Shui, W Q.]]
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[[Category: Rao ZH]]
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[[Category: Sun, Y N.]]
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[[Category: Shui WQ]]
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[[Category: Wang, J.]]
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[[Category: Sun YN]]
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[[Category: Drug discovery]]
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[[Category: Wang J]]
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[[Category: Drug resistant]]
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[[Category: Hydrolase]]
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[[Category: Ndm-1]]
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[[Category: New delhi metallo-beta-lactamase 1]]
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Current revision

Crystal structure of New Delhi Metallo-beta-lactamase (NDM-1)

PDB ID 3s0z

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