1ixs

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[[Image:1ixs.png|left|200px]]
 
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{{STRUCTURE_1ixs| PDB=1ixs | SCENE= }}
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==Structure of RuvB complexed with RuvA domain III==
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<StructureSection load='1ixs' size='340' side='right'caption='[[1ixs]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ixs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IXS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ixs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ixs OCA], [https://pdbe.org/1ixs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ixs RCSB], [https://www.ebi.ac.uk/pdbsum/1ixs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ixs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RUVA_THET8 RUVA_THET8] The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/1ixs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ixs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We present the X-ray structure of the RuvA-RuvB complex, which plays a crucial role in ATP-dependent branch migration. Two RuvA tetramers form the symmetric and closed octameric shell, where four RuvA domain IIIs spring out in the two opposite directions to be individually caught by a single RuvB. The binding of domain III deforms the protruding beta hairpin in the N-terminal domain of RuvB and thereby appears to induce a functional and less symmetric RuvB hexameric ring. The model of the RuvA-RuvB junction DNA ternary complex, constructed by fitting the X-ray structure into the averaged electron microscopic images of the RuvA-RuvB junction, appears to be more compatible with the branch migration mode of a fixed RuvA-RuvB interaction than with a rotational interaction mode.
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===Structure of RuvB complexed with RuvA domain III===
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Crystal structure of the RuvA-RuvB complex: a structural basis for the Holliday junction migrating motor machinery.,Yamada K, Miyata T, Tsuchiya D, Oyama T, Fujiwara Y, Ohnishi T, Iwasaki H, Shinagawa H, Ariyoshi M, Mayanagi K, Morikawa K Mol Cell. 2002 Sep;10(3):671-81. PMID:12408833<ref>PMID:12408833</ref>
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{{ABSTRACT_PUBMED_12408833}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1ixs" style="background-color:#fffaf0;"></div>
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[[1ixs]] is a 2 chain structure of [[Helicase]] with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IXS OCA].
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==See Also==
==See Also==
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*[[Helicase|Helicase]]
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*[[Helicase 3D structures|Helicase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:012408833</ref><references group="xtra"/>
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__TOC__
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[[Category: Adenosinetriphosphatase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Ariyoshi, M.]]
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[[Category: Ariyoshi M]]
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[[Category: Fujiwara, Y.]]
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[[Category: Fujiwara Y]]
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[[Category: Iwasaki, H.]]
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[[Category: Iwasaki H]]
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[[Category: Mayanagi, K.]]
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[[Category: Mayanagi K]]
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[[Category: Miyata, T.]]
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[[Category: Miyata T]]
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[[Category: Morikawa, K.]]
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[[Category: Morikawa K]]
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[[Category: Ohnishi, T.]]
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[[Category: Ohnishi T]]
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[[Category: Oyama, T.]]
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[[Category: Oyama T]]
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[[Category: Shinagawa, H.]]
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[[Category: Shinagawa H]]
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[[Category: Tsuchiya, D.]]
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[[Category: Tsuchiya D]]
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[[Category: Yamada, K.]]
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[[Category: Yamada K]]
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[[Category: Aaa-atpase domain]]
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[[Category: Complex with nucleotide]]
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[[Category: Heterodimeric protein complex]]
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[[Category: Hydrolase]]
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Current revision

Structure of RuvB complexed with RuvA domain III

PDB ID 1ixs

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