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1fhz

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[[Image:1fhz.gif|left|200px]]<br /><applet load="1fhz" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fhz, resolution 2.20&Aring;" />
 
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'''PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION'''<br />
 
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==Overview==
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==PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION==
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The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.
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<StructureSection load='1fhz' size='340' side='right'caption='[[1fhz]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1fhz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FHZ FirstGlance]. <br>
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1FHZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=PSO:'>PSO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHZ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PSO:4-HYDROXYMETHYL-4,5,8-TRIMETHYLPSORALEN'>PSO</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fhz OCA], [https://pdbe.org/1fhz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fhz RCSB], [https://www.ebi.ac.uk/pdbsum/1fhz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fhz ProSAT]</span></td></tr>
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The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11302703 11302703]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Alberti, M.]]
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</StructureSection>
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[[Category: Eichman, B F.]]
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[[Category: Large Structures]]
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[[Category: Hearst, J E.]]
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[[Category: Alberti M]]
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[[Category: Ho, P S.]]
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[[Category: Eichman BF]]
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[[Category: Mooers, B H.M.]]
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[[Category: Hearst JE]]
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[[Category: PSO]]
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[[Category: Ho PS]]
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[[Category: cross-linked dna]]
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[[Category: Mooers BHM]]
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[[Category: dna-drug complex]]
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[[Category: holliday junction]]
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[[Category: psoralen]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:38:50 2008''
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Current revision

PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION

PDB ID 1fhz

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