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1fml

From Proteopedia

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[[Image:1fml.gif|left|200px]]<br /><applet load="1fml" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fml, resolution 2.75&Aring;" />
 
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'''CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP==
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We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase.
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<StructureSection load='1fml' size='340' side='right'caption='[[1fml]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1fml]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FML FirstGlance]. <br>
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1FML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=A3P:'>A3P</scene> and <scene name='pdbligand=RTL:'>RTL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FML OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=RTL:RETINOL'>RTL</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fml OCA], [https://pdbe.org/1fml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fml RCSB], [https://www.ebi.ac.uk/pdbsum/1fml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fml ProSAT]</span></td></tr>
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A helical lid converts a sulfotransferase to a dehydratase., Pakhomova S, Kobayashi M, Buck J, Newcomer ME, Nat Struct Biol. 2001 May;8(5):447-51. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11323722 11323722]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/Q26490_SPOFR Q26490_SPOFR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fm/1fml_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fml ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Spodoptera frugiperda]]
[[Category: Spodoptera frugiperda]]
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[[Category: Buck, J.]]
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[[Category: Buck J]]
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[[Category: Kobayashi, M.]]
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[[Category: Kobayashi M]]
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[[Category: Newcomer, M E.]]
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[[Category: Newcomer ME]]
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[[Category: Pakhomova, S.]]
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[[Category: Pakhomova S]]
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[[Category: A3P]]
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[[Category: CA]]
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[[Category: RTL]]
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[[Category: 5'-diphosphate]]
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[[Category: adenosine 3']]
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[[Category: dehydratase]]
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[[Category: retinol]]
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[[Category: sulfotransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:40:16 2008''
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Current revision

CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP

PDB ID 1fml

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