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2fff

From Proteopedia

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[[Image:2fff.png|left|200px]]
 
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{{STRUCTURE_2fff| PDB=2fff | SCENE= }}
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==Open Form of a Class A Transpeptidase Domain==
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<StructureSection load='2fff' size='340' side='right'caption='[[2fff]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2fff]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FFF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.23&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fff OCA], [https://pdbe.org/2fff PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fff RCSB], [https://www.ebi.ac.uk/pdbsum/2fff PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fff ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O70038_STREE O70038_STREE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ff/2fff_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fff ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The class A PBP1b from Streptococcus pneumoniae is responsible for glycosyltransferase and transpeptidase (TP) reactions, forming the peptidoglycan of the bacterial cell wall. The enzyme has been produced in a stable, soluble form and undergoes time-dependent proteolysis to leave an intact TP domain. Crystals of this TP domain were obtained, diffracting to 2.2 A resolution, and the structure was solved by using molecular replacement. Analysis of the structure revealed an "open" active site, with important conformational differences to the previously determined "closed" apoenzyme. The active-site nucleophile, Ser460, is in an orientation that allows for acylation by beta-lactams. Consistent with the productive conformation of the conserved active-site catalytic residues, adjacent loops show only minor deviation from those of known acyl-enzyme structures. These findings are discussed in the context of enzyme functionality and the possible conformational sampling of PBP1b between active and inactive states.
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===Open Form of a Class A Transpeptidase Domain===
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Structural analysis of an "open" form of PBP1B from Streptococcus pneumoniae.,Lovering AL, De Castro L, Lim D, Strynadka NC Protein Sci. 2006 Jul;15(7):1701-9. Epub 2006 Jun 2. PMID:16751607<ref>PMID:16751607</ref>
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{{ABSTRACT_PUBMED_16751607}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2fff" style="background-color:#fffaf0;"></div>
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[[2fff]] is a 2 chain structure of [[Penicillin-binding protein]] with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFF OCA].
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==See Also==
==See Also==
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*[[Penicillin-binding protein|Penicillin-binding protein]]
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*[[Penicillin-binding protein 3D structures|Penicillin-binding protein 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:016751607</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptococcus pneumoniae]]
[[Category: Streptococcus pneumoniae]]
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[[Category: Lovering, A L.]]
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[[Category: Lovering AL]]
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[[Category: Strynadka, N C.J.]]
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[[Category: Strynadka NCJ]]
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[[Category: Membrane protein]]
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[[Category: Transferase]]
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[[Category: Transpeptidase fold]]
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Current revision

Open Form of a Class A Transpeptidase Domain

PDB ID 2fff

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