1wnu

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[[Image:1wnu.png|left|200px]]
 
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{{STRUCTURE_1wnu| PDB=1wnu | SCENE= }}
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==Structure of Archaeal Trans-Editing Protein AlaX in complex with L-serine==
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<StructureSection load='1wnu' size='340' side='right'caption='[[1wnu]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1wnu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WNU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wnu OCA], [https://pdbe.org/1wnu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wnu RCSB], [https://www.ebi.ac.uk/pdbsum/1wnu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wnu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALAXS_PYRHO ALAXS_PYRHO] Functions in trans to edit the amino acid moiety from mischarged charged Ser-tRNA(Ala). Has little activity against Gly-tRNA(Ala).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wn/1wnu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wnu ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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AlaX is the homologue of the class II alanyl-tRNA synthetase editing domain and has been shown to exhibit autonomous editing activity against mischarged tRNA(Ala). Here, we present the structures of AlaX from the archaeon Pyrococcus horikoshii in apo form, complexed with zinc, and with noncognate amino acid l-serine and zinc. Together with mutational analysis, we demonstrated that the conserved Thr-30 hydroxyl group located near the beta-methylene of the bound serine is responsible for the discrimination of noncognate serine from cognate alanine, based on their chemical natures. Furthermore, we confirmed that the conserved Gln-584 in alanyl-tRNA synthetase, which corresponds to Thr-30 of AlaX, is also critical for discrimination. These observations strongly suggested conservation of the chemical discrimination among trans- and cis-editing of tRNA(Ala).
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===Structure of Archaeal Trans-Editing Protein AlaX in complex with L-serine===
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Molecular basis of alanine discrimination in editing site.,Sokabe M, Okada A, Yao M, Nakashima T, Tanaka I Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11669-74. Epub 2005 Aug 8. PMID:16087889<ref>PMID:16087889</ref>
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{{ABSTRACT_PUBMED_16087889}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1wnu" style="background-color:#fffaf0;"></div>
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[[1wnu]] is a 2 chain structure of [[Aminoacyl tRNA Synthetase]] with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNU OCA].
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==See Also==
==See Also==
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*[[Aminoacyl tRNA Synthetase|Aminoacyl tRNA Synthetase]]
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:016087889</ref><ref group="xtra">PMID:018172502</ref><references group="xtra"/>
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__TOC__
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[[Category: Pyrococcus horikoshii]]
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</StructureSection>
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[[Category: Nakashima, T.]]
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[[Category: Large Structures]]
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[[Category: Okada, A.]]
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[[Category: Pyrococcus horikoshii OT3]]
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[[Category: Sokabe, M.]]
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[[Category: Nakashima T]]
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[[Category: Tanaka, I.]]
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[[Category: Okada A]]
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[[Category: Yao, M.]]
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[[Category: Sokabe M]]
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[[Category: Hydrolase]]
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[[Category: Tanaka I]]
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[[Category: Yao M]]

Current revision

Structure of Archaeal Trans-Editing Protein AlaX in complex with L-serine

PDB ID 1wnu

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