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1x9m

From Proteopedia

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[[Image:1x9m.png|left|200px]]
 
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{{STRUCTURE_1x9m| PDB=1x9m | SCENE= }}
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==T7 DNA polymerase in complex with an N-2-acetylaminofluorene-adducted DNA==
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<StructureSection load='1x9m' size='340' side='right'caption='[[1x9m]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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===T7 DNA polymerase in complex with an N-2-acetylaminofluorene-adducted DNA===
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1x9m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X9M FirstGlance]. <br>
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{{ABSTRACT_PUBMED_15528277}}
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2DT:3-DEOXYTHYMIDINE-5-MONOPHOSPHATE'>2DT</scene>, <scene name='pdbligand=8FG:N-(5-PHOSPHO-2-DEOXYGUANOSIN-8-YL)-2-ACETYLAMINOFLUORENE'>8FG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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==About this Structure==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x9m OCA], [https://pdbe.org/1x9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x9m RCSB], [https://www.ebi.ac.uk/pdbsum/1x9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x9m ProSAT]</span></td></tr>
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[[1x9m]] is a 4 chain structure of [[DNA polymerase]] and [[Thioredoxin]] with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X9M OCA].
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/THIO_ECOLI THIO_ECOLI] Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x9/1x9m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x9m ConSurf].
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<div style="clear:both"></div>
==See Also==
==See Also==
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*[[DNA polymerase|DNA polymerase]]
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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*[[Thioredoxin|Thioredoxin]]
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*[[Thioredoxin 3D structures|Thioredoxin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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<ref group="xtra">PMID:015528277</ref><references group="xtra"/>
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[[Category: DNA-directed DNA polymerase]]
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[[Category: Enterobacteria phage t7]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Dutta, S.]]
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[[Category: Escherichia phage T7]]
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[[Category: Dzantiev, L.]]
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[[Category: Large Structures]]
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[[Category: Ellenberger, T.]]
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[[Category: Dutta S]]
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[[Category: Johnson, D.]]
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[[Category: Dzantiev L]]
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[[Category: Li, Y.]]
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[[Category: Ellenberger T]]
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[[Category: Richardson, C C.]]
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[[Category: Johnson D]]
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[[Category: Romano, L J.]]
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[[Category: Li Y]]
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[[Category: Dna ploymerase]]
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[[Category: Richardson CC]]
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[[Category: Mutagenesis]]
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[[Category: Romano LJ]]
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[[Category: N-2-acetylaminofluorene]]
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[[Category: Replication block]]
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[[Category: Transferase-electron transport-dna complex]]
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Current revision

T7 DNA polymerase in complex with an N-2-acetylaminofluorene-adducted DNA

PDB ID 1x9m

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