3gi8

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[[Image:3gi8.png|left|200px]]
 
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{{STRUCTURE_3gi8| PDB=3gi8 | SCENE= }}
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==Crystal Structure of ApcT K158A Transporter Bound to 7F11 Monoclonal Fab Fragment==
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<StructureSection load='3gi8' size='340' side='right'caption='[[3gi8]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3gi8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GI8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GI8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.59&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gi8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gi8 OCA], [https://pdbe.org/3gi8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gi8 RCSB], [https://www.ebi.ac.uk/pdbsum/3gi8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gi8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Y609_METJA Y609_METJA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gi/3gi8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gi8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Amino acid, polyamine, and organocation (APC) transporters are secondary transporters that play essential roles in nutrient uptake, neurotransmitter recycling, ionic homeostasis, and regulation of cell volume. Here, we present the crystal structure of apo-ApcT, a proton-coupled broad-specificity amino acid transporter, at 2.35 A resolution. The structure contains 12 transmembrane helices, with the first 10 consisting of an inverted structural repeat of 5 transmembrane helices like LeuT. The ApcT structure reveals an inward-facing, apo state and an amine moiety of Lys158 located in a position equivalent to the Na2 ion of LeuT. We propose that Lys158 is central to proton-coupled transport and that the amine group serves the same functional role as the Na2 ion in LeuT, thus demonstrating common principles among proton- and sodium-coupled transporters.
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===Crystal Structure of ApcT K158A Transporter Bound to 7F11 Monoclonal Fab Fragment===
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Structure and Mechanism of a Na+ Independent Amino Acid Transporter.,Shaffer PL, Goehring A, Shankaranarayanan A, Gouaux E Science. 2009 Jul 22. PMID:19608859<ref>PMID:19608859</ref>
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{{ABSTRACT_PUBMED_19608859}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3gi8" style="background-color:#fffaf0;"></div>
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[[3gi8]] is a 3 chain structure of [[Monoclonal Antibody]] with sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GI8 OCA].
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==See Also==
==See Also==
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*[[Monoclonal Antibody|Monoclonal Antibody]]
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019608859</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Goehring, A S.]]
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[[Category: Goehring AS]]
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[[Category: Gouaux, E.]]
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[[Category: Gouaux E]]
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[[Category: NYCOMPS, New York Consortium on Membrane Protein Structure.]]
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[[Category: Shaffer PL]]
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[[Category: Shaffer, P L.]]
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[[Category: Shankaranarayanan A]]
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[[Category: Shankaranarayanan, A.]]
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[[Category: Antibody]]
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[[Category: Cell membrane]]
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[[Category: Membrane]]
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[[Category: Membrane protein]]
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[[Category: New york consortium on membrane protein structure]]
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[[Category: Nycomp]]
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[[Category: Protein structure initiative]]
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[[Category: Psi-2]]
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[[Category: Structural genomic]]
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[[Category: Transmembrane]]
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[[Category: Transport protein]]
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[[Category: Transporter]]
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Current revision

Crystal Structure of ApcT K158A Transporter Bound to 7F11 Monoclonal Fab Fragment

PDB ID 3gi8

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