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1fvn

From Proteopedia

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[[Image:1fvn.gif|left|200px]]<br /><applet load="1fvn" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fvn" />
 
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'''[ALA31, AIB32]-NEUROPEPTIDE Y'''<br />
 
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==Overview==
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==[ALA31, AIB32]-NEUROPEPTIDE Y==
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The first Y(5) receptor-selective analog of neuropeptide Y (NPY), [Ala(31),Aib(32)]NPY, has been developed and biologically characterized. Using competition binding assays on cell lines that express different Y receptors, we determined the affinity of this analog to be 6 nm at the human Y(5) receptor, &gt;500 nm at the Y(1) and Y(2) receptors, and &gt;1000 nm at the Y(4) receptor. Activity studies performed in vitro using a cAMP enzyme immunoassay, and in vivo using food intake studies in rats, showed that the peptide acted as an agonist. Further peptides obtained by the combination of the Ala(31)-Aib(32) motif with chimeric peptides containing segments of NPY and pancreatic polypeptide displayed the same selectivity and even higher affinity (up to 0.2 nm) for the Y(5) receptor. In vivo administration of the new Y(5) receptor-selective agonists significantly stimulated feeding in rats. The NMR solution structures of NPY and [Ala(31),Aib(32)]NPY showed a different conformation in the C-terminal region, where the alpha-helix of NPY was substituted by a more flexible, 3(10)-helical turn structure.
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<StructureSection load='1fvn' size='340' side='right'caption='[[1fvn]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1fvn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FVN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FVN FirstGlance]. <br>
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1FVN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FVN OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AIB:ALPHA-AMINOISOBUTYRIC+ACID'>AIB</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fvn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fvn OCA], [https://pdbe.org/1fvn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fvn RCSB], [https://www.ebi.ac.uk/pdbsum/1fvn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fvn ProSAT]</span></td></tr>
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The first selective agonist for the neuropeptide YY5 receptor increases food intake in rats., Cabrele C, Langer M, Bader R, Wieland HA, Doods HN, Zerbe O, Beck-Sickinger AG, J Biol Chem. 2000 Nov 17;275(46):36043-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10944518 10944518]
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</table>
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[[Category: Single protein]]
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== Function ==
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[[Category: Bader, R.]]
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[https://www.uniprot.org/uniprot/NPY_PIG NPY_PIG] NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
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[[Category: Beck-Sickinger, A G.]]
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== Evolutionary Conservation ==
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[[Category: Zerbe, O.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: NH2]]
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Check<jmol>
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[[Category: 310-helix]]
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<jmolCheckbox>
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[[Category: alpha]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fv/1fvn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:43:05 2008''
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fvn ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Sus scrofa]]
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[[Category: Bader R]]
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[[Category: Beck-Sickinger AG]]
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[[Category: Zerbe O]]

Current revision

[ALA31, AIB32]-NEUROPEPTIDE Y

PDB ID 1fvn

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