1fxl

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[[Image:1fxl.gif|left|200px]]<br /><applet load="1fxl" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fxl, resolution 1.8&Aring;" />
 
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'''CRYSTAL STRUCTURE OF HUD AND AU-RICH ELEMENT OF THE C-FOS RNA'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF HUD AND AU-RICH ELEMENT OF THE C-FOS RNA==
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Hu proteins bind to adenosine-uridine (AU)-rich elements (AREs) in the 3' untranslated regions of many short-lived mRNAs, thereby stabilizing them. Here we report the crystal structures of the first two RNA recognition motif (RRM) domains of the HuD protein in complex with an 11-nucleotide fragment of a class I ARE (the c-fos ARE; to 1.8 A), and with an 11-nucleotide fragment of a class II ARE (the tumor necrosis factor alpha ARE; to 2.3 A). These structures reveal a consensus RNA recognition sequence that suggests a preference for pyrimidine-rich sequences and a requirement for a central uracil residue in the clustered AUUUA repeats found in class II AREs. Comparison to structures of other RRM domain-nucleic acid complexes reveals two base recognition pockets in all the structures that interact with bases using residues in conserved ribonucleoprotein motifs and at the C-terminal ends of RRM domains. Different conformations of nucleic acid can be bound by RRM domains by using different combinations of base recognition pockets and multiple RRM domains.
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<StructureSection load='1fxl' size='340' side='right'caption='[[1fxl]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==Disease==
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<table><tr><td colspan='2'>[[1fxl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FXL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FXL FirstGlance]. <br>
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Known diseases associated with this structure: Neuropathy, paraneoplastic sensory OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=168360 168360]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fxl OCA], [https://pdbe.org/1fxl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fxl RCSB], [https://www.ebi.ac.uk/pdbsum/1fxl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fxl ProSAT]</span></td></tr>
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==About this Structure==
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</table>
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1FXL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FXL OCA].
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== Function ==
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[https://www.uniprot.org/uniprot/ELAV4_HUMAN ELAV4_HUMAN] May play a role in neuron-specific RNA processing. Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity). Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF and FOS mRNA.<ref>PMID:7898713</ref> <ref>PMID:10710437</ref>
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==Reference==
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== Evolutionary Conservation ==
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Structural basis for recognition of AU-rich element RNA by the HuD protein., Wang X, Tanaka Hall TM, Nat Struct Biol. 2001 Feb;8(2):141-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11175903 11175903]
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fx/1fxl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fxl ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hall, T M.T.]]
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[[Category: Hall TMT]]
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[[Category: Wang, X.]]
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[[Category: Wang X]]
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[[Category: au-rich element]]
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[[Category: hud]]
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[[Category: protein-rna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:43:41 2008''
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Current revision

CRYSTAL STRUCTURE OF HUD AND AU-RICH ELEMENT OF THE C-FOS RNA

PDB ID 1fxl

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