3k24

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[[Image:3k24.png|left|200px]]
 
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{{STRUCTURE_3k24| PDB=3k24 | SCENE= }}
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==Crystal structure of mature apo-Cathepsin L C25A mutant in complex with Gln-Leu-Ala peptide==
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<StructureSection load='3k24' size='340' side='right'caption='[[3k24]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3k24]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K24 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K24 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k24 OCA], [https://pdbe.org/3k24 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k24 RCSB], [https://www.ebi.ac.uk/pdbsum/3k24 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k24 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CATL1_HUMAN CATL1_HUMAN] Important for the overall degradation of proteins in lysosomes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k2/3k24_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3k24 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Proteolysis of eukaryotic histone tails has emerged as an important factor in the modulation of cell-cycle progression and cellular differentiation. The recruitment of lysosomal cathepsin L to the nucleus where it mediates proteolysis of the mouse histone H3 tail has been described recently. Here, we report the three-dimensional crystal structures of a mature, inactive mutant of human cathepsin L alone and in complex with a peptide derived from histone H3. Canonical substrate-cathepsin L interactions are observed in the complex between the protease and the histone H3 peptide. Systematic analysis of the impact of posttranslational modifications at histone H3 on substrate selectivity suggests cathepsin L to be highly accommodating of all modified peptides. This is the first report of cathepsin L-histone H3 interaction and the first structural description of cathepsin L in complex with a substrate.
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===Crystal structure of mature apo-Cathepsin L C25A mutant in complex with Gln-Leu-Ala peptide===
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Structural basis for the recognition and cleavage of histone H3 by cathepsin L.,Adams-Cioaba MA, Krupa JC, Xu C, Mort JS, Min J Nat Commun. 2011 Feb;2:197. PMID:21326229<ref>PMID:21326229</ref>
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{{ABSTRACT_PUBMED_21326229}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3k24" style="background-color:#fffaf0;"></div>
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[[3k24]] is a 4 chain structure of [[Cathepsin]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K24 OCA].
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==See Also==
==See Also==
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*[[Cathepsin|Cathepsin]]
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*[[Cathepsin 3D structures|Cathepsin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021326229</ref><references group="xtra"/>
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__TOC__
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[[Category: Cathepsin L]]
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Adams-Cioaba, M A.]]
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[[Category: Large Structures]]
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[[Category: Arrowsmith, C H.]]
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[[Category: Adams-Cioaba MA]]
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[[Category: Bochkarev, A.]]
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[[Category: Arrowsmith CH]]
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[[Category: Bountra, C.]]
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[[Category: Bochkarev A]]
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[[Category: Edwards, A M.]]
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[[Category: Bountra C]]
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[[Category: Krupa, J C.]]
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[[Category: Edwards AM]]
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[[Category: Min, J.]]
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[[Category: Krupa JC]]
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[[Category: Mort, J S.]]
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[[Category: Min J]]
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[[Category: SGC, Structural Genomics Consortium.]]
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[[Category: Mort JS]]
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[[Category: Weigelt, J.]]
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[[Category: Weigelt J]]
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[[Category: Co-crystal]]
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[[Category: Hydrolase]]
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[[Category: Protease]]
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[[Category: Sgc]]
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[[Category: Structural genomic]]
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[[Category: Structural genomics consortium]]
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[[Category: Substrate]]
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Current revision

Crystal structure of mature apo-Cathepsin L C25A mutant in complex with Gln-Leu-Ala peptide

PDB ID 3k24

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