|
|
| (15 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:1fzp.gif|left|200px]]<br /><applet load="1fzp" size="350" color="white" frame="true" align="right" spinBox="true"
| + | REMOVED: The PDB entry 1fzp was removed. |
| - | caption="1fzp, resolution 2.95Å" />
| + | |
| - | '''CRYSTAL STRUCTURES OF SARA: A PLEIOTROPIC REGULATOR OF VIRULENCE GENES IN S. AUREUS'''<br />
| + | |
| - | | + | |
| - | ==Overview==
| + | |
| - | Staphylococcus aureus is a major human pathogen, the potency of which can be attributed to the regulated expression of an impressive array of virulence determinants. A key pleiotropic transcriptional regulator of these virulence factors is SarA, which is encoded by the sar (staphylococcal accessory regulator) locus. SarA was characterized initially as an activator of a second virulence regulatory locus, agr, through its interaction with a series of heptad repeats (AGTTAAG) within the agr promoter. Subsequent DNA-binding studies have revealed that SarA binds readily to multiple AT-rich sequences of variable lengths. Here we describe the crystal structure of SarA and a SarA-DNA complex at resolutions of 2.50 A and 2.95 A, respectively. SarA has a fold consisting of a four-helix core region and 'inducible regions' comprising a beta-hairpin and a carboxy-terminal loop. On binding DNA, the inducible regions undergo marked conformational changes, becoming part of extended and distorted alpha-helices, which encase the DNA. SarA recognizes an AT-rich site in which the DNA is highly overwound and adopts a D-DNA-like conformation by indirect readout. These structures thus provide insight into SarA-mediated transcription regulation.
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1FZP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FZP OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Crystal structures of SarA, a pleiotropic regulator of virulence genes in S. aureus., Schumacher MA, Hurlburt BK, Brennan RG, Nature. 2001 Jan 11;409(6817):215-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11196648 11196648]
| + | |
| - | [[Category: Single protein]]
| + | |
| - | [[Category: Staphylococcus aureus]]
| + | |
| - | [[Category: Brennan, R G.]]
| + | |
| - | [[Category: Hurlburt, B.]]
| + | |
| - | [[Category: Schumacher, M A.]]
| + | |
| - | [[Category: CA]]
| + | |
| - | [[Category: coil to helix]]
| + | |
| - | [[Category: conformational changes]]
| + | |
| - | [[Category: d-dna]]
| + | |
| - | [[Category: indirect readout]]
| + | |
| - | [[Category: protein-dna complex]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:44:24 2008''
| + | |
