1jfi

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (03:44, 6 June 2025) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1jfi.png|left|200px]]
 
-
{{STRUCTURE_1jfi| PDB=1jfi | SCENE= }}
+
==Crystal Structure of the NC2-TBP-DNA Ternary Complex==
 +
<StructureSection load='1jfi' size='340' side='right'caption='[[1jfi]], [[Resolution|resolution]] 2.62&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1jfi]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The July 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''TATA-Binding Protein'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_7 10.2210/rcsb_pdb/mom_2005_7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JFI FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.62&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jfi OCA], [https://pdbe.org/1jfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jfi RCSB], [https://www.ebi.ac.uk/pdbsum/1jfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jfi ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1jfi TOPSAN]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/NC2A_HUMAN NC2A_HUMAN] The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own.<ref>PMID:8670811</ref> <ref>PMID:8608938</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jf/1jfi_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jfi ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The X-ray structure of a ternary complex of Negative Cofactor 2 (NC2), the TATA box binding protein (TBP), and DNA has been determined at 2.6 A resolution. The N termini of NC2 alpha and beta resemble histones H2A and H2B, respectively, and form a heterodimer that binds to the bent DNA double helix on the underside of the preformed TBP-DNA complex via electrostatic interactions. NC2beta contributes to inhibition of TATA-dependent transcription through interactions of its C-terminal alpha helix with a conserved hydrophobic feature on the upper surface of TBP, which in turn positions the penultimate alpha helix of NC2beta to block recognition of the TBP-DNA complex by transcription factor IIB. Further regulatory implications of the NC2 heterodimer structure are discussed.
-
===Crystal Structure of the NC2-TBP-DNA Ternary Complex===
+
Crystal structure of negative cofactor 2 recognizing the TBP-DNA transcription complex.,Kamada K, Shu F, Chen H, Malik S, Stelzer G, Roeder RG, Meisterernst M, Burley SK Cell. 2001 Jul 13;106(1):71-81. PMID:11461703<ref>PMID:11461703</ref>
-
{{ABSTRACT_PUBMED_11461703}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 1jfi" style="background-color:#fffaf0;"></div>
-
[[1jfi]] is a 5 chain structure of [[TATA-Binding Protein]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The July 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''TATA-Binding Protein'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_7 10.2210/rcsb_pdb/mom_2005_7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JFI OCA].
+
==See Also==
==See Also==
-
*[[TATA-Binding Protein|TATA-Binding Protein]]
+
*[[TATA-binding protein 3D structures|TATA-binding protein 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:011461703</ref><references group="xtra"/>
+
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
 +
[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: TATA-Binding Protein]]
[[Category: TATA-Binding Protein]]
-
[[Category: Burley, S K.]]
+
[[Category: Burley SK]]
-
[[Category: Chen, H.]]
+
[[Category: Chen H]]
-
[[Category: Kamada, K.]]
+
[[Category: Kamada K]]
-
[[Category: Malik, S.]]
+
[[Category: Malik S]]
-
[[Category: Meisterernst, M.]]
+
[[Category: Meisterernst M]]
-
[[Category: NYSGXRC, New York SGX Research Center for Structural Genomics.]]
+
[[Category: Roeder RG]]
-
[[Category: Roeder, R G.]]
+
[[Category: Shu F]]
-
[[Category: Shu, F.]]
+
[[Category: Stelzer G]]
-
[[Category: Stelzer, G.]]
+
-
[[Category: H2a/h2b]]
+
-
[[Category: Histone]]
+
-
[[Category: Nc2]]
+
-
[[Category: Negative cofactor]]
+
-
[[Category: New york sgx research center for structural genomic]]
+
-
[[Category: Nysgxrc]]
+
-
[[Category: Protein structure initiative]]
+
-
[[Category: Psi]]
+
-
[[Category: Structural genomic]]
+
-
[[Category: Tata-dna]]
+
-
[[Category: Tbp]]
+
-
[[Category: Transcription initiation]]
+
-
[[Category: Transcription-dna complex]]
+

Current revision

Crystal Structure of the NC2-TBP-DNA Ternary Complex

PDB ID 1jfi

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jfi"
Personal tools