1qe0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "1qe0" [edit=sysop:move=sysop])
Current revision (07:15, 30 October 2024) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1qe0.png|left|200px]]
 
-
{{STRUCTURE_1qe0| PDB=1qe0 | SCENE= }}
+
==CRYSTAL STRUCTURE OF APO S. AUREUS HISTIDYL-TRNA SYNTHETASE==
 +
<StructureSection load='1qe0' size='340' side='right'caption='[[1qe0]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1qe0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QE0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QE0 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qe0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qe0 OCA], [https://pdbe.org/1qe0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qe0 RCSB], [https://www.ebi.ac.uk/pdbsum/1qe0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qe0 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SYH_STAAU SYH_STAAU]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qe/1qe0_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qe0 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The crystal structure of the Staphylococcus aureus histidyl-tRNA synthetase apoprotein has been determined at 2.7 A resolution. Several important loops in the active site either become disordered or adopt very different conformations compared to their ligand-bound states. These include the histidine A motif (Arg257-Tyr262) that is essential for substrate recognition, a loop (Gly52-Lys62) that seems to control the communication between the histidine and ATP binding sites, the motif 2 loop (Glu114-Arg120) that binds ATP, and the insertion domain that is likely to bind tRNA. These ligand-induced structural changes are supported by fluorescence experiments, which also suggest highly cooperative dynamics. A dynamic and cooperative active site is most likely necessary for the proper functioning of the histidyl-tRNA synthetase, and suggests a novel mechanism for improving charging fidelity.
-
===CRYSTAL STRUCTURE OF APO S. AUREUS HISTIDYL-TRNA SYNTHETASE===
+
Cooperative structural dynamics and a novel fidelity mechanism in histidyl-tRNA synthetases.,Qiu X, Janson CA, Blackburn MN, Chhohan IK, Hibbs M, Abdel-Meguid SS Biochemistry. 1999 Sep 21;38(38):12296-304. PMID:10493797<ref>PMID:10493797</ref>
-
{{ABSTRACT_PUBMED_10493797}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 1qe0" style="background-color:#fffaf0;"></div>
-
[[1qe0]] is a 2 chain structure of [[Aminoacyl tRNA Synthetase]] with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QE0 OCA].
+
==See Also==
==See Also==
-
*[[Aminoacyl tRNA Synthetase|Aminoacyl tRNA Synthetase]]
+
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:010493797</ref><references group="xtra"/>
+
__TOC__
-
[[Category: Histidine--tRNA ligase]]
+
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
-
[[Category: Abdel-Meguid, S S.]]
+
[[Category: Abdel-Meguid SS]]
-
[[Category: Blackburn, M N.]]
+
[[Category: Blackburn MN]]
-
[[Category: Chohan, I K.]]
+
[[Category: Chohan IK]]
-
[[Category: Hibbs, M.]]
+
[[Category: Hibbs M]]
-
[[Category: Janson, C A.]]
+
[[Category: Janson CA]]
-
[[Category: Qiu, X.]]
+
[[Category: Qiu X]]
-
[[Category: Beta sheet]]
+
-
[[Category: Class ii trna synthetase]]
+
-
[[Category: Ligase]]
+

Current revision

CRYSTAL STRUCTURE OF APO S. AUREUS HISTIDYL-TRNA SYNTHETASE

PDB ID 1qe0

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qe0"
Personal tools