2kh9

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[[Image:2kh9.png|left|200px]]
 
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{{STRUCTURE_2kh9| PDB=2kh9 | SCENE= }}
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==Solution structure of yeast Prp24-RRM2 bound to a fragment of U6 RNA==
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<StructureSection load='2kh9' size='340' side='right'caption='[[2kh9]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2kh9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KH9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KH9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kh9 OCA], [https://pdbe.org/2kh9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kh9 RCSB], [https://www.ebi.ac.uk/pdbsum/2kh9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kh9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PRP24_YEAST PRP24_YEAST] Binds preferentially to the U4/U6 hybrid snRNAs. Can stimulate the annealing of U4 and U6. Could participate in both the formation and disassembly of the U4/U6 hybrid during splicing.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kh/2kh9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kh9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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U6 RNA plays a critical role in pre-mRNA splicing. Assembly of U6 into the spliceosome requires a significant structural rearrangement and base-pairing with U4 RNA. In the yeast Saccharomyces cerevisiae, this process requires the essential splicing factor Prp24. We present the characterization and structure of a complex containing one of Prp24's four RNA recognition motif (RRM) domains, RRM2, and a fragment of U6 RNA. NMR methods were used to identify the preferred U6 binding sequence of RRM2 (5'-GAGA-3'), measure the affinity of the interaction, and solve the structure of RRM2 bound to the hexaribonucleotide AGAGAU. Interdomain contacts observed between RRM2 and RRM3 in a crystal structure of the free protein are not detectable in solution. A structural model of RRM1 and RRM2 bound to a longer segment of U6 RNA is presented, and a partial mechanism for Prp24's annealing activity is proposed.
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===Solution structure of yeast Prp24-RRM2 bound to a fragment of U6 RNA===
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Structure and functional implications of a complex containing a segment of U6 RNA bound by a domain of Prp24.,Martin-Tumasz S, Reiter NJ, Brow DA, Butcher SE RNA. 2010 Apr;16(4):792-804. Epub 2010 Feb 24. PMID:20181740<ref>PMID:20181740</ref>
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{{ABSTRACT_PUBMED_20181740}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2kh9" style="background-color:#fffaf0;"></div>
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[[2kh9]] is a 2 chain structure of [[Pre-mRNA-splicing factor]] with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KH9 OCA].
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==See Also==
==See Also==
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*[[Pre-mRNA-splicing factor|Pre-mRNA-splicing factor]]
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*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020181740</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Butcher, S E.]]
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[[Category: Butcher SE]]
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[[Category: Martin-Tumasz, S A.]]
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[[Category: Martin-Tumasz SA]]
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[[Category: Mrna processing]]
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[[Category: Mrna splicing]]
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[[Category: Nucleus]]
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[[Category: Phosphoprotein]]
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[[Category: Protein/rna]]
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[[Category: Rna-binding]]
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[[Category: Rrm]]
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[[Category: Snrnp]]
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[[Category: Splicing]]
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[[Category: Splicing-rna complex]]
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Current revision

Solution structure of yeast Prp24-RRM2 bound to a fragment of U6 RNA

PDB ID 2kh9

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