2kh9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2kh9" [edit=sysop:move=sysop])
Current revision (09:38, 22 May 2024) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2kh9.png|left|200px]]
 
-
{{STRUCTURE_2kh9| PDB=2kh9 | SCENE= }}
+
==Solution structure of yeast Prp24-RRM2 bound to a fragment of U6 RNA==
 +
<StructureSection load='2kh9' size='340' side='right'caption='[[2kh9]]' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2kh9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KH9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KH9 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kh9 OCA], [https://pdbe.org/2kh9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kh9 RCSB], [https://www.ebi.ac.uk/pdbsum/2kh9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kh9 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PRP24_YEAST PRP24_YEAST] Binds preferentially to the U4/U6 hybrid snRNAs. Can stimulate the annealing of U4 and U6. Could participate in both the formation and disassembly of the U4/U6 hybrid during splicing.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kh/2kh9_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kh9 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
U6 RNA plays a critical role in pre-mRNA splicing. Assembly of U6 into the spliceosome requires a significant structural rearrangement and base-pairing with U4 RNA. In the yeast Saccharomyces cerevisiae, this process requires the essential splicing factor Prp24. We present the characterization and structure of a complex containing one of Prp24's four RNA recognition motif (RRM) domains, RRM2, and a fragment of U6 RNA. NMR methods were used to identify the preferred U6 binding sequence of RRM2 (5'-GAGA-3'), measure the affinity of the interaction, and solve the structure of RRM2 bound to the hexaribonucleotide AGAGAU. Interdomain contacts observed between RRM2 and RRM3 in a crystal structure of the free protein are not detectable in solution. A structural model of RRM1 and RRM2 bound to a longer segment of U6 RNA is presented, and a partial mechanism for Prp24's annealing activity is proposed.
-
===Solution structure of yeast Prp24-RRM2 bound to a fragment of U6 RNA===
+
Structure and functional implications of a complex containing a segment of U6 RNA bound by a domain of Prp24.,Martin-Tumasz S, Reiter NJ, Brow DA, Butcher SE RNA. 2010 Apr;16(4):792-804. Epub 2010 Feb 24. PMID:20181740<ref>PMID:20181740</ref>
-
{{ABSTRACT_PUBMED_20181740}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 2kh9" style="background-color:#fffaf0;"></div>
-
[[2kh9]] is a 2 chain structure of [[Pre-mRNA-splicing factor]] with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KH9 OCA].
+
==See Also==
==See Also==
-
*[[Pre-mRNA-splicing factor|Pre-mRNA-splicing factor]]
+
*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:020181740</ref><references group="xtra"/>
+
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Butcher, S E.]]
+
[[Category: Butcher SE]]
-
[[Category: Martin-Tumasz, S A.]]
+
[[Category: Martin-Tumasz SA]]
-
[[Category: Mrna processing]]
+
-
[[Category: Mrna splicing]]
+
-
[[Category: Nucleus]]
+
-
[[Category: Phosphoprotein]]
+
-
[[Category: Protein/rna]]
+
-
[[Category: Rna-binding]]
+
-
[[Category: Rrm]]
+
-
[[Category: Snrnp]]
+
-
[[Category: Splicing]]
+
-
[[Category: Splicing-rna complex]]
+

Current revision

Solution structure of yeast Prp24-RRM2 bound to a fragment of U6 RNA

PDB ID 2kh9

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2kh9"
Personal tools