1g6q

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[[Image:1g6q.gif|left|200px]]<br /><applet load="1g6q" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g6q, resolution 2.90&Aring;" />
 
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'''CRYSTAL STRUCTURE OF YEAST ARGININE METHYLTRANSFERASE, HMT1'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF YEAST ARGININE METHYLTRANSFERASE, HMT1==
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Protein methylation at arginines is ubiquitous in eukaryotes and affects signal transduction, gene expression and protein sorting. Hmt1/Rmt1, the major arginine methyltransferase in yeast, catalyzes methylation of arginine residues in several mRNA-binding proteins and facilitates their export from the nucleus. We now report the crystal structure of Hmt1 at 2.9 A resolution. Hmt1 forms a hexamer with approximate 32 symmetry. The surface of the oligomer is dominated by large acidic cavities at the dimer interfaces. Mutation of dimer contact sites eliminates activity of Hmt1 both in vivo and in vitro. Mutating residues in the acidic cavity significantly reduces binding and methylation of the substrate Npl3.
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<StructureSection load='1g6q' size='340' side='right'caption='[[1g6q]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1g6q]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G6Q FirstGlance]. <br>
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1G6Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6Q OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6q OCA], [https://pdbe.org/1g6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g6q RCSB], [https://www.ebi.ac.uk/pdbsum/1g6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g6q ProSAT]</span></td></tr>
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==Reference==
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</table>
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The structure and oligomerization of the yeast arginine methyltransferase, Hmt1., Weiss VH, McBride AE, Soriano MA, Filman DJ, Silver PA, Hogle JM, Nat Struct Biol. 2000 Dec;7(12):1165-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11101900 11101900]
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== Function ==
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[https://www.uniprot.org/uniprot/HMT1_YEAST HMT1_YEAST] Methylates arginines in a variety of RNA-binding proteins. Methylates NOP3. Can catalyze both the mono- and asymmetric dimethylation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g6/1g6q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g6q ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Filman DJ]]
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[[Category: Filman, D J.]]
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[[Category: Hogle JM]]
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[[Category: Hogle, J M.]]
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[[Category: McBride AE]]
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[[Category: McBride, A E.]]
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[[Category: Silver PA]]
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[[Category: Silver, P A.]]
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[[Category: Soriano MA]]
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[[Category: Soriano, M A.]]
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[[Category: Weiss VH]]
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[[Category: Weiss, V H.]]
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[[Category: beta-barrel]]
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[[Category: dimer]]
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[[Category: hexamer]]
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[[Category: mixed alpha-beta]]
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[[Category: sam-binding domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:46:43 2008''
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Current revision

CRYSTAL STRUCTURE OF YEAST ARGININE METHYLTRANSFERASE, HMT1

PDB ID 1g6q

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