3ts6

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[[Image:3ts6.png|left|200px]]
 
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{{STRUCTURE_3ts6| PDB=3ts6 | SCENE= }}
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==Crystal structure of M-PMV DUTPASE relaxed end-product (dUMP) complex==
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<StructureSection load='3ts6' size='340' side='right'caption='[[3ts6]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ts6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mason-Pfizer_monkey_virus Mason-Pfizer monkey virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TS6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TS6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.84&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ts6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ts6 OCA], [https://pdbe.org/3ts6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ts6 RCSB], [https://www.ebi.ac.uk/pdbsum/3ts6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ts6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PRO_MPMV PRO_MPMV] Matrix protein. Nucleocapsid protein p14: Nucleocapsid protein. Capsid protein. The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell.[PROSITE-ProRule:PRU00275]<ref>PMID:9636364</ref> The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell.[PROSITE-ProRule:PRU00275]<ref>PMID:9636364</ref> Enhances the activity of the reverse transcriptase. May be part of the mature RT.<ref>PMID:22171253</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Deoxyuridine 5'-triphosphate nucleotidohydrolase from Mason-Pfizer monkey retrovirus (M-PMV dUTPase) is a betaretroviral member of the dUTPase enzyme family. In the mature M-PMV virion, this enzyme is present as the C-terminal domain of the fusion protein nucleocapsid-dUTPase. The homotrimeric organization characteristic of dUTPases is retained in this bifunctional fusion protein. The fusion protein supposedly plays a role in adequate localization of dUTPase activity in the vicinity of nucleic acids during reverse transcription and integration. Here, the nucleocapsid-free dUTPase (48 426 Da) was cocrystallized with a dUTP substrate analogue using the hanging-drop vapour-diffusion method. The obtained crystals belong to the primitive hexagonal space group P6(3), with unit-cell parameters a = 60.6, b = 60.6, c = 63.6 angstroms, alpha = 90, beta = 90, gamma = 120 degrees. Native and PtCl4-derivative data sets were collected using synchrotron radiation to 1.75 and 2.3 angstroms, respectively. Phasing was successfully performed by isomorphous replacement combined with anomalous scattering.
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===Crystal structure of M-PMV DUTPASE relaxed end-product (dUMP) complex===
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Crystallization and preliminary X-ray studies of dUTPase from Mason-Pfizer monkey retrovirus.,Barabas O, Nemeth V, Vertessy BG Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt, 4):399-401. Epub 2006 Mar 25. PMID:016582495<ref>PMID:016582495</ref>
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{{ABSTRACT_PUBMED_16582495}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3ts6" style="background-color:#fffaf0;"></div>
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[[3ts6]] is a 1 chain structure of [[Deoxyuridine 5'-triphosphate nucleotidohydrolase]] with sequence from [http://en.wikipedia.org/wiki/Mason-pfizer_monkey_virus Mason-pfizer monkey virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TS6 OCA].
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==See Also==
==See Also==
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*[[Deoxyuridine 5'-triphosphate nucleotidohydrolase|Deoxyuridine 5'-triphosphate nucleotidohydrolase]]
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*[[DUTPase 3D structures|DUTPase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:016582495</ref><references group="xtra"/>
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__TOC__
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[[Category: Mason-pfizer monkey virus]]
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</StructureSection>
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[[Category: DUTP diphosphatase]]
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[[Category: Large Structures]]
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[[Category: Barabas, O.]]
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[[Category: Mason-Pfizer monkey virus]]
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[[Category: Nemeth, V.]]
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[[Category: Barabas O]]
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[[Category: Vertessy, G B.]]
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[[Category: Nemeth V]]
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[[Category: Hydrolase]]
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[[Category: Vertessy GB]]
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[[Category: Jelly roll]]
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Current revision

Crystal structure of M-PMV DUTPASE relaxed end-product (dUMP) complex

PDB ID 3ts6

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