1g8l

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[[Image:1g8l.jpg|left|200px]]<br /><applet load="1g8l" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g8l, resolution 1.95&Aring;" />
 
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'''CRYSTAL STRUCTURE OF ESCHERICHIA COLI MOEA'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF ESCHERICHIA COLI MOEA==
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BACKGROUND: Molybdenum cofactor (Moco) biosynthesis is an evolutionarily conserved pathway present in archaea, eubacteria, and eukaryotes. In humans, genetic abnormalities in the biosynthetic pathway result in Moco deficiency, which is accompanied by severe neurological symptoms and death shortly after birth. The Escherichia coli MoeA and MogA proteins are involved in the final step of Moco biosynthesis: the incorporation of molybdenum into molybdopterin (MPT), the organic pyranopterin moiety of Moco. RESULTS: The crystal structure of E. coli MoeA has been refined at 2 A resolution and reveals that the highly elongated MoeA monomer consists of four clearly separated domains, one of which is structurally related to MogA, indicating a divergent evolutionary relationship between both proteins. The active form of MoeA is a dimer, and a putative active site appears to be localized to a cleft formed between domain II of the first monomer and domains III and IV of the second monomer. CONCLUSIONS: In eukaryotes, MogA and MoeA are fused into a single polypeptide chain. The corresponding mammalian protein gephyrin has also been implicated in the anchoring of glycinergic receptors to the cytoskeleton at inhibitory synapses. Based on the structures of MoeA and MogA, gephyrin is surmised to be a highly organized molecule containing at least five domains. This multidomain arrangement could provide a structural basis for its functional diversity. The oligomeric states of MoeA and MogA suggest how gephyrin could assemble into a hexagonal scaffold at inhibitory synapses.
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<StructureSection load='1g8l' size='340' side='right'caption='[[1g8l]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1g8l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G8L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G8L FirstGlance]. <br>
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1G8L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G8L OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g8l OCA], [https://pdbe.org/1g8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g8l RCSB], [https://www.ebi.ac.uk/pdbsum/1g8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g8l ProSAT]</span></td></tr>
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The crystal structure of Escherichia coli MoeA and its relationship to the multifunctional protein gephyrin., Xiang S, Nichols J, Rajagopalan KV, Schindelin H, Structure. 2001 Apr 4;9(4):299-310. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11525167 11525167]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MOEA_ECOLI MOEA_ECOLI] Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP.<ref>PMID:15632135</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g8/1g8l_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g8l ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Nichols, J.]]
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[[Category: Nichols J]]
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[[Category: Rajagopalan, K V.]]
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[[Category: Rajagopalan KV]]
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[[Category: Schindelin, H.]]
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[[Category: Schindelin H]]
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[[Category: Xiang, S.]]
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[[Category: Xiang S]]
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[[Category: GOL]]
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[[Category: molybdenum cofactor biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:47:18 2008''
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Current revision

CRYSTAL STRUCTURE OF ESCHERICHIA COLI MOEA

PDB ID 1g8l

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