1g9s

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[[Image:1g9s.jpg|left|200px]]<br /><applet load="1g9s" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g9s, resolution 2.8&Aring;" />
 
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'''CRYSTAL STRUCTURE OF A COMPLEX BETWEEN E.COLI HPRT AND IMP'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF A COMPLEX BETWEEN E.COLI HPRT AND IMP==
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Crystal structures have been determined for free Escherichia coli hypoxanthine phosphoribosyltransferase (HPRT) (2.9 A resolution) and for the enzyme in complex with the reaction products, inosine 5'-monophosphate (IMP) and guanosine 5'-monophosphate (GMP) (2.8 A resolution). Of the known 6-oxopurine phosphoribosyltransferase (PRTase) structures, E. coli HPRT is most similar in structure to that of Tritrichomonas foetus HGXPRT, with a rmsd for 150 Calpha atoms of 1.0 A. Comparison of the free and product bound structures shows that the side chain of Phe156 and the polypeptide backbone in this vicinity move to bind IMP or GMP. A nonproline cis peptide bond, also found in some other 6-oxopurine PRTases, is observed between Leu46 and Arg47 in both the free and complexed structures. For catalysis to occur, the 6-oxopurine PRTases have a requirement for divalent metal ion, usually Mg(2+) in vivo. In the free structure, a Mg(2+) is coordinated to the side chains of Glu103 and Asp104. This interaction may be important for stabilization of the enzyme before catalysis. E. coli HPRT is unique among the known 6-oxopurine PRTases in that it exhibits a marked preference for hypoxanthine as substrate over both xanthine and guanine. The structures suggest that its substrate specificity is due to the modes of binding of the bases. In E. coli HPRT, the carbonyl oxygen of Asp163 would likely form a hydrogen bond with the 2-exocyclic nitrogen of guanine (in the HPRT-guanine-PRib-PP-Mg(2+) complex). However, hypoxanthine does not have a 2-exocyclic atom and the HPRT-IMP structure suggests that hypoxanthine is likely to occupy a different position in the purine-binding pocket.
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<StructureSection load='1g9s' size='340' side='right'caption='[[1g9s]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1g9s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G9S FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene>, <scene name='pdbligand=N:ANY+5-MONOPHOSPHATE+NUCLEOTIDE'>N</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g9s OCA], [https://pdbe.org/1g9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g9s RCSB], [https://www.ebi.ac.uk/pdbsum/1g9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g9s ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HPRT_ECOLI HPRT_ECOLI] Acts preferentially on hypoxanthine; has very low activity towards guanine. Inactive towards xanthine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g9/1g9s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g9s ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1G9S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=IMP:'>IMP</scene> and <scene name='pdbligand=N:'>N</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Hypoxanthine_phosphoribosyltransferase Hypoxanthine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.8 2.4.2.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G9S OCA].
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*[[Phosphoribosyltransferase 3D structures|Phosphoribosyltransferase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structures of free, IMP-, and GMP-bound Escherichia coli hypoxanthine phosphoribosyltransferase., Guddat LW, Vos S, Martin JL, Keough DT, de Jersey J, Protein Sci. 2002 Jul;11(7):1626-38. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12070315 12070315]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Hypoxanthine phosphoribosyltransferase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Guddat LW]]
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[[Category: Guddat, L W.]]
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[[Category: Keough DT]]
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[[Category: Jersey, J de.]]
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[[Category: Martin JL]]
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[[Category: Keough, D T.]]
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[[Category: Vos S]]
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[[Category: Martin, J L.]]
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[[Category: De Jersey J]]
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[[Category: Vos, S.]]
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[[Category: IMP]]
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[[Category: N]]
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[[Category: crystal structure]]
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[[Category: enzymology]]
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[[Category: phosphoribosyltransferases]]
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[[Category: protein chemistry]]
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[[Category: purine salvage]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:47:47 2008''
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Current revision

CRYSTAL STRUCTURE OF A COMPLEX BETWEEN E.COLI HPRT AND IMP

PDB ID 1g9s

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