2ddc

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[[Image:2ddc.png|left|200px]]
 
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{{STRUCTURE_2ddc| PDB=2ddc | SCENE= }}
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==Unique behavior of a histidine responsible for an engineered green-to-red photoconversion process==
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<StructureSection load='2ddc' size='340' side='right'caption='[[2ddc]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ddc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dipsastraea_favus Dipsastraea favus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DDC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DDC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ddc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ddc OCA], [https://pdbe.org/2ddc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ddc RCSB], [https://www.ebi.ac.uk/pdbsum/2ddc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ddc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q53UG8_DIPFA Q53UG8_DIPFA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dd/2ddc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ddc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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KikGR is a fluorescent protein engineered to display green-to-red photoconvertibility that is induced by irradiation with ultraviolet or violet light. Similar to Kaede and EosFP, two naturally occurring photoconvertible proteins, KikGR contains a His(62)-Tyr(63)-Gly(64) tripeptide sequence, which forms a green chromophore that can be photoconverted to a red one via formal beta-elimination and subsequent extension of a pi-conjugated system. Using a crystallizable variant of KikGR, we determined the structures of both the green and red state at 1.55 A resolution. The double bond between His(62)-C(alpha) and His(62)-C(beta) in the red chromophore is in a cis configuration, indicating that rotation along the His(62) C(alpha)-C(beta) bond occurs following cleavage of the His(62) N(alpha)-C(alpha) bond. This structural rearrangement provides evidence that the beta-elimination reaction governing the green-to-red photoconversion of KikGR follows an E1 (elimination, unimolecular) mechanism.
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===Unique behavior of a histidine responsible for an engineered green-to-red photoconversion process===
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The E1 mechanism in photo-induced beta-elimination reactions for green-to-red conversion of fluorescent proteins.,Tsutsui H, Shimizu H, Mizuno H, Nukina N, Furuta T, Miyawaki A Chem Biol. 2009 Nov 25;16(11):1140-7. PMID:19942137<ref>PMID:19942137</ref>
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{{ABSTRACT_PUBMED_019942137}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2ddc" style="background-color:#fffaf0;"></div>
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[[2ddc]] is a 2 chain structure of [[Alyssa Marsico/Sandbox 1]], [[Devon McCarthy/Sandbox 1]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]] with sequence from [http://en.wikipedia.org/wiki/Favia_favus Favia favus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DDC OCA].
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==See Also==
==See Also==
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*[[Alyssa Marsico/Sandbox 1|Alyssa Marsico/Sandbox 1]]
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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*[[Devon McCarthy/Sandbox 1|Devon McCarthy/Sandbox 1]]
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== References ==
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*[[Green Fluorescent Protein|Green Fluorescent Protein]]
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<references/>
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*[[Sandbox104|Sandbox104]]
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__TOC__
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*[[User:Joanne Lau/Sandbox 5|User:Joanne Lau/Sandbox 5]]
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</StructureSection>
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[[Category: Dipsastraea favus]]
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==Reference==
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[[Category: Large Structures]]
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<ref group="xtra">PMID:019942137</ref><references group="xtra"/>
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[[Category: Miyawaki A]]
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[[Category: Favia favus]]
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[[Category: Nukina N]]
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[[Category: Miyawaki, A.]]
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[[Category: Shimizu H]]
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[[Category: Nukina, N.]]
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[[Category: Tsutsui H]]
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[[Category: Shimizu, H.]]
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[[Category: Tsutsui, H.]]
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[[Category: Luminescent protein]]
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[[Category: Photoconversion]]
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[[Category: Red state]]
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Current revision

Unique behavior of a histidine responsible for an engineered green-to-red photoconversion process

PDB ID 2ddc

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