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1ghf

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[[Image:1ghf.gif|left|200px]]<br /><applet load="1ghf" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ghf, resolution 2.7&Aring;" />
 
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'''ANTI-ANTI-IDIOTYPE GH1002 FAB FRAGMENT'''<br />
 
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==Overview==
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==ANTI-ANTI-IDIOTYPE GH1002 FAB FRAGMENT==
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The structure of the Fab fragment of the mouse anti-anti-idiotypic monoclonal antibody (mAb) GH1002 was solved by X-ray crystallography. mAb GH1002 was elicited with the syngeneic anti-idiotype mAb MK2-23 which mimics the determinant defined by anti-human high molecular weight-melanoma associated antigen (HMW-MAA) mAb 763.74. The Fab fragments of mAb GH1002 exist in the crystal as dimers related by crystallographic 2-fold axes. The interface between dyad-related Fab fragments is formed primarily by interaction of the hypervariable loops of one with the other. The self-interaction of Fab fragments of anti-anti-idiotypic mAb GH1002 through their combining sites is extremely tight and intricate, closely resembling that observed in structures of id-anti-id complexes, and comparable in terms of total contact area, charge complementarity, and number of hydrogen bonds. The self-complementarity of the antibody observed here could be coincidental and thus reflect some non-specific binding capability. It might, on the other hand, be immunologically relevant and exemplify a certain degree of evolved self complementarity characteristic of antibodies participating in idiotypic cascades.
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<StructureSection load='1ghf' size='340' side='right'caption='[[1ghf]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ghf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GHF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ghf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ghf OCA], [https://pdbe.org/1ghf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ghf RCSB], [https://www.ebi.ac.uk/pdbsum/1ghf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ghf ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gh/1ghf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ghf ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1GHF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GHF OCA].
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Sandbox 20009|Sandbox 20009]]
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==Reference==
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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Crystal structure of an anti-anti-idiotype shows it to be self-complementary., Ban N, Day J, Wang X, Ferrone S, McPherson A, J Mol Biol. 1996 Feb 2;255(4):617-27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8568901 8568901]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Ban N]]
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[[Category: Ban, N.]]
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[[Category: Day J]]
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[[Category: Day, J.]]
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[[Category: Ferrone S]]
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[[Category: Ferrone, S.]]
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[[Category: McPherson A]]
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[[Category: McPherson, A.]]
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[[Category: Wang X]]
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[[Category: Wang, X.]]
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[[Category: antibody fab fragment]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:50:04 2008''
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Current revision

ANTI-ANTI-IDIOTYPE GH1002 FAB FRAGMENT

PDB ID 1ghf

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