1go8

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[[Image:1go8.jpg|left|200px]]<br /><applet load="1go8" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1go8, resolution 2.00&Aring;" />
 
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'''THE METZINCIN'S METHIONINE: PRTC M226L MUTANT'''<br />
 
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==Overview==
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==The metzincin's methionine: PrtC M226L mutant==
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Serralysins are a family of metalloproteases secreted by Gram-negative bacteria into the medium in the form of inactive zymogens. Usually, all serralysin secretors have on the same operon a gene coding for a periplasmic 10-kDa protein, which is an inhibitor of the secreted protease. The recent characterization of the inhibitor of the alkaline protease from Pseudomonas aeruginosa revealed a surprisingly low dissociation constant of 4 pm, contrary to earlier studies on homologous systems, where inhibition constants in the microm range were reported. To approach a more accurate understanding, the crystal structure of the complex between inhibitor and protease from P. aeruginosa was determined at 1.74 A resolution and refined to R(free) = 0.204. The structure reported here shows clearly that the N terminus of the inhibitor forms a coordinative bond to the catalytic Zn(2+) ion with a nitrogen-zinc distance of 2.17 A. We conclude that this interaction adds substantially to the complex stability and show also that similar interactions are found in other metzincin-inhibitor complexes.
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<StructureSection load='1go8' size='340' side='right'caption='[[1go8]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1go8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GO8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GO8 FirstGlance]. <br>
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1GO8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Po4+Binding+Site+For+Chain+P'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GO8 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1go8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1go8 OCA], [https://pdbe.org/1go8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1go8 RCSB], [https://www.ebi.ac.uk/pdbsum/1go8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1go8 ProSAT]</span></td></tr>
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Crystal structure of a complex between Pseudomonas aeruginosa alkaline protease and its cognate inhibitor: inhibition by a zinc-NH2 coordinative bond., Hege T, Feltzer RE, Gray RD, Baumann U, J Biol Chem. 2001 Sep 14;276(37):35087-92. Epub 2001 Jul 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11445573 11445573]
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</table>
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[[Category: Erwinia chrysanthemi]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/PRTC_DICCH PRTC_DICCH]
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[[Category: Baumann, U.]]
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== Evolutionary Conservation ==
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[[Category: CA]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: PO4]]
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Check<jmol>
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[[Category: ZN]]
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<jmolCheckbox>
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[[Category: hydrolase]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/go/1go8_consurf.spt"</scriptWhenChecked>
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[[Category: metalloprotease]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: protease]]
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:52:12 2008''
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1go8 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Dickeya chrysanthemi]]
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[[Category: Large Structures]]
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[[Category: Baumann U]]

Current revision

The metzincin's methionine: PrtC M226L mutant

PDB ID 1go8

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