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3dtp

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[[Image:3dtp.png|left|200px]]
 
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{{STRUCTURE_3dtp| PDB=3dtp | SCENE= }}
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==Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP==
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<SX load='3dtp' size='340' side='right' viewer='molstar' caption='[[3dtp]], [[Resolution|resolution]] 20.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3dtp]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Avicularia_avicularia Avicularia avicularia] and [https://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DTP FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1i84|1i84]], [[2fxm|2fxm]], [[1b7t|1b7t]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MYH7, MYHCB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK]), MYL6 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dtp OCA], [https://pdbe.org/3dtp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dtp RCSB], [https://www.ebi.ac.uk/pdbsum/3dtp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dtp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/MYH11_CHICK MYH11_CHICK]] Muscle contraction. [[https://www.uniprot.org/uniprot/MYL6_CHICK MYL6_CHICK]] Regulatory light chain of myosin. Does not bind calcium.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dt/3dtp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dtp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Muscle contraction involves the interaction of the myosin heads of the thick filaments with actin subunits of the thin filaments. Relaxation occurs when this interaction is blocked by molecular switches on these filaments. In many muscles, myosin-linked regulation involves phosphorylation of the myosin regulatory light chains (RLCs). Electron microscopy of vertebrate smooth muscle myosin molecules (regulated by phosphorylation) has provided insight into the relaxed structure, revealing that myosin is switched off by intramolecular interactions between its two heads, the free head and the blocked head. Three-dimensional reconstruction of frozen-hydrated specimens revealed that this asymmetric head interaction is also present in native thick filaments of tarantula striated muscle. Our goal in this study was to elucidate the structural features of the tarantula filament involved in phosphorylation-based regulation. A new reconstruction revealed intra- and intermolecular myosin interactions in addition to those seen previously. To help interpret the interactions, we sequenced the tarantula RLC and fitted an atomic model of the myosin head that included the predicted RLC atomic structure and an S2 (subfragment 2) crystal structure to the reconstruction. The fitting suggests one intramolecular interaction, between the cardiomyopathy loop of the free head and its own S2, and two intermolecular interactions, between the cardiac loop of the free head and the essential light chain of the blocked head and between the Leu305-Gln327 interaction loop of the free head and the N-terminal fragment of the RLC of the blocked head. These interactions, added to those previously described, would help switch off the thick filament. Molecular dynamics simulations suggest how phosphorylation could increase the helical content of the RLC N-terminus, weakening these interactions, thus releasing both heads and activating the thick filament.
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===Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP===
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Three-dimensional reconstruction of tarantula myosin filaments suggests how phosphorylation may regulate myosin activity.,Alamo L, Wriggers W, Pinto A, Bartoli F, Salazar L, Zhao FQ, Craig R, Padron R J Mol Biol. 2008 Dec 26;384(4):780-97. Epub 2008 Oct 14. PMID:18951904<ref>PMID:18951904</ref>
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{{ABSTRACT_PUBMED_18951904}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3dtp" style="background-color:#fffaf0;"></div>
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[[3dtp]] is a 6 chain structure of [[Myosin]] with sequence from [http://en.wikipedia.org/wiki/Avicularia_avicularia Avicularia avicularia], [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Gallus_gallus,_homo_sapiens Gallus gallus, homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DTP OCA].
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==See Also==
==See Also==
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*[[Myosin|Myosin]]
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*[[Myosin 3D Structures|Myosin 3D Structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:018951904</ref><ref group="xtra">PMID:012798686</ref><ref group="xtra">PMID:017095604</ref><ref group="xtra">PMID:010338210</ref><references group="xtra"/>
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__TOC__
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</SX>
[[Category: Avicularia avicularia]]
[[Category: Avicularia avicularia]]
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[[Category: Gallus gallus]]
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[[Category: Chick]]
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[[Category: Gallus gallus, homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Alamo, L.]]
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[[Category: Alamo, L]]
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[[Category: Bartoli, F.]]
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[[Category: Bartoli, F]]
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[[Category: Craig, R.]]
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[[Category: Craig, R]]
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[[Category: Padron, R.]]
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[[Category: Padron, R]]
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[[Category: Pinto, A.]]
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[[Category: Pinto, A]]
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[[Category: Salazar, L.]]
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[[Category: Salazar, L]]
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[[Category: Wriggers, W.]]
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[[Category: Wriggers, W]]
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[[Category: Zhao, F Q.]]
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[[Category: Zhao, F Q]]
[[Category: Coiled-coil]]
[[Category: Coiled-coil]]
[[Category: Contractile protein]]
[[Category: Contractile protein]]

Current revision

Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP

3dtp, resolution 20.00Å

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